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Literature summary extracted from

  • Habison, A.; Kubicek, C.P.; Röhr, M.
    Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger (1983), Biochem. J., 209, 669-676.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Aspergillus niger
2.7.1.11 ADP at high fructose 6-phosphate concentration, inhibits at low fructose 6-phosphate concentration Aspergillus niger
2.7.1.11 AMP activation at low fructose 6-phosphate concentrations, inhibition at high fructose 6-phosphate concentrations Aspergillus niger
2.7.1.11 D-glucose 6-phosphate 4.8 mM, 1.5fold increase in activity at 2 mM fructose 6-phosphate, 2fold increase at 4 mM fructose 6-phosphate, in vitro Aspergillus niger
2.7.1.11 additional information not activated by 2-oxoglutarate Aspergillus niger
2.7.1.11 NH4+ NH4+ increases the affinity for fructose 6-phosphate, counteracts the inhibition by ATP Aspergillus niger

General Stability

EC Number General Stability Organism
2.7.1.11 dilution inactivates Aspergillus niger
2.7.1.11 fructose 6-phosphate stabilizes, not 1 mM ATP or 50% v/v glycerol Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ADP kinetics Aspergillus niger
2.7.1.11 AMP at high fructose 6-phosphate concentrations, activates at low fructose 6-phosphate concentrations Aspergillus niger
2.7.1.11 ATP synergistic with citrate Aspergillus niger
2.7.1.11 citrate strong inhibition; synergistic with ATP; synergistic with NH4+; synergistic with phosphate and AMP Aspergillus niger
2.7.1.11 additional information not inhibited by cAMP Aspergillus niger
2.7.1.11 phosphoenolpyruvate
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.08
-
 ATP pH 7.8, 30°C Aspergillus niger
2.7.1.11 0.08
-
 GTP pH 7.8, 30°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Aspergillus niger
-
-
-
2.7.1.11 Aspergillus niger B60
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 partial Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 6.1
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Aspergillus niger ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Aspergillus niger B60 ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Aspergillus niger GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Aspergillus niger B60 GDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 30
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7.6 8
-
 Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.11 0.15
-
 phosphoenolpyruvate pH 7.8, 30°C Aspergillus niger
2.7.1.11 0.25
-
 citrate pH 7.8, 30°C, at 0.1 mM ATP Aspergillus niger
2.7.1.11 0.4
-
 citrate pH 7.8, 30°C, at 0.5 mM ATP Aspergillus niger