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Literature summary extracted from

  • Allen, C.M.; Muth, J.D.; Gildersleeve, N.
    Extraction and detergent/lipid activation of dolichol kinase (1982), Biochim. Biophys. Acta, 712, 33-41.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.108 dimyristoylphosphatidylcholine stimulatory in 1:1 ratio with deoxycholate Bos taurus
2.7.1.108 additional information sodium dodecylsulfate is ineffective in stimulating enzymic activity Bos taurus
2.7.1.108 Triton X-100
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.108 Ca2+ above 6 mM Bos taurus
2.7.1.108 Co2+ above 6 mM Bos taurus
2.7.1.108 Mn2+ above 6 mM Bos taurus
2.7.1.108 additional information Mg2+ is not inhibitory Bos taurus
2.7.1.108 Zn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.108 0.15
-
 CTP pH 7.5, 35°C, in deoxycholate/dimyristoylphosphatidylcholine 1:1 Bos taurus
2.7.1.108 0.55
-
 CTP pH 7.5, 35°C, in Triton X-100/dimyristoylphosphatidylcholine 1:1 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.108 membrane
-
 Bos taurus 16020
-
2.7.1.108 microsome
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.108 Ca2+
-
 Bos taurus
2.7.1.108 Co2+ divalent cation required for activity, Mg2+, Ca2+ and Co2+ are equally effective Bos taurus
2.7.1.108 Mg2+ enzyme is dependent on divalent cations Bos taurus
2.7.1.108 additional information Zn2+ is not effective Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.108 CTP + dolichol Bos taurus
-
 CDP + dolichyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.108 Bos taurus
-
 bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.108 partially Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.108 liver
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.108 -20°C, stored frozen as pellet for months with little loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.108 CTP + dolichol
-
 Bos taurus CDP + dolichyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.108 8.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.108 6.6 10.2 broad pH-activity profile with good activity observed from pH 7.0-10.0, about half-maximal activity at pH 6.6 and 10.2 Bos taurus