Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wissing, J.; Heim, S.; Wagner, K.G.
    Diacylglycerol kinase from suspension cultured plant cells (1989), Plant Physiol., 90, 1546-1551.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 deoxycholate purified enzyme is completely devoid of activity without addition of phospholipid or deoxycholate Catharanthus roseus
2.7.1.107 Phospholipid purified enzyme is completely devoid of activity without addition of phospholipid or deoxycholate Catharanthus roseus

General Stability

EC Number General Stability Organism
2.7.1.107 enzyme is unstable in membrane extract, upon storage overnight at 4°C 90% of the activity is lost Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 Ca2+
-
 Catharanthus roseus
2.7.1.107 Co2+
-
 Catharanthus roseus
2.7.1.107 deoxycholate 1 mM, about 50% inhibition Catharanthus roseus
2.7.1.107 Triton X-100 0.5 mM, 50% inhibition Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.1
-
 ATP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.25
-
 1,2-diacyl-sn-glycerol pH 7.5, 25°C Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane bound to Catharanthus roseus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+ divalent cation is required for activity. Mg2+ is most effective, maximal activation at about 10 mM Catharanthus roseus
2.7.1.107 Mn2+ slight activation at 5 mM Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 51000
-
 1 * 51000, SDS-PAGE Catharanthus roseus
2.7.1.107 68000
-
 less than, sucrose density gradient centrifugation Catharanthus roseus
2.7.1.107 250000
-
 gel filtration Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107
-
 Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 cell suspension culture
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 0.231
-
-
 Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
 Catharanthus roseus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + sn-1,2-dioleoylglycerol
-
 Catharanthus roseus ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 monomer 1 * 51000, SDS-PAGE Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 7
-
-
 Catharanthus roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.107 6.5 7 rapid decrease of activity below pH 6.5, pH 7: optimum Catharanthus roseus