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Literature summary extracted from

  • Harmsen, H.J.M.; Kubicek-Pranz, E.M.; Röhr, M.; Visser, J.; Kubicek, C.P.
    Regulation of 6-phosphofructo-2-kinase from the citric-acid-accumulating fungus Aspergillus niger (1992), Appl. Microbiol. Biotechnol., 37, 784-788.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.105 very unstable upon purification Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 AMP weak Aspergillus niger
2.7.1.105 citrate weak Aspergillus niger
2.7.1.105 diphosphate not phosphate Aspergillus niger
2.7.1.105 additional information no inhibition by protein kinase C; phosphorylation by cAMP-dependent protein kinase, but no inhibition Aspergillus niger
2.7.1.105 phosphoenolpyruvate weak Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.6
-
 beta-D-fructose 6-phosphate
-
 Aspergillus niger
2.7.1.105 0.7
-
 ATP
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+
-
 Aspergillus niger
2.7.1.105 phosphate requirement Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase, but no inhibition Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 additional information
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
2.7.1.105 4°C, t1/2: 4 h Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Aspergillus niger ADP + beta-D-fructose 2,6-bisphosphate
-
 ?