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Literature summary extracted from

  • Ferro, A.J.; Barrett, A.; Shapiro, S.K.
    5-Methylthioribose kinase. A new enzyme involved in the formation of methionine from 5-methylthioribose (1978), J. Biol. Chem., 253, 6021-6025.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.100 dithiothreitol required for activity, maximal activity at 20 mM Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.100 ADP 52% inhibition at equimolar concentrations with ATP Klebsiella aerogenes
2.7.1.100 CTP in the presence of ATP Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.100 0.0081
-
 5-methylthioribose pH 7.3, 37°C Klebsiella aerogenes
2.7.1.100 0.074
-
 ATP pH 7.3, 37°C Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.100 Mg2+ maximal activity at 5 mM Klebsiella aerogenes
2.7.1.100 Mg2+ divalent metal ion is strictely required for activity, Mg2+ is most effective Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose Klebsiella aerogenes may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine ADP + 5-methylthioribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.100 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.100 ammonium sulfate, Sephadex G-200, DEAE-cellulose Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.100 0.028
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.100 -20°C, 20% glycerol, at least 5 mg/ml, stable Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose no activity with GTP and UTP Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-methylthioribose may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 CTP + 5-methylthioribose 20% of activity with ATP Klebsiella aerogenes CDP + 5-methylthioribose 1-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.100 7.3
-
-
 Klebsiella aerogenes