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Literature summary extracted from

  • Steinb÷ck, F.; Choojun, S.; Held, I.; Roehr, M.; Kubicek, C.P.
    Characterization and regulatory properties of a single hexokinase from the citric acid accumulating fungus Aspergillus niger (1994), Biochim. Biophys. Acta, 1200, 215-223.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP
-
 Aspergillus niger
2.7.1.1 citrate noncompetitive to glucose and ATP Aspergillus niger
2.7.1.1 Mg2+ enzyme is inhibited by an excess of free divalent metal ion, Mg2+ or Mn2+ Aspergillus niger
2.7.1.1 Mn2+ enzyme is inhibited by an excess of free divalent metal ion, Mg2+ or Mn2+ Aspergillus niger
2.7.1.1 additional information not inhibited by D-glucose 6-phosphate Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.023
-
 D-glucose
-
 Aspergillus niger
2.7.1.1 0.54
-
 ATP
-
 Aspergillus niger
2.7.1.1 29
-
 D-fructose
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ Aspergillus niger
2.7.1.1 Mn2+ affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 50000
-
 2 * 50000, SDS-PAGE Aspergillus niger
2.7.1.1 100000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Aspergillus niger
-
 strain ATCC 11414
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 about 600fold Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 mycelium glucose-grown Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 35
-
 D-fructose Aspergillus niger
2.7.1.1 40
-
 D-glucose Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Aspergillus niger ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP
-
 Aspergillus niger ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Aspergillus niger ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP
-
 Aspergillus niger ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 additional information not: D-galactose Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 dimer 2 * 50000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 30
-
 assay at Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 additional information
-
 high activity over a broad pH-range Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP affinity for ATP depends on the presence of a divalent metal ion, Mg2+ or Mn2+ Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.15
-
 citrate
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Aspergillus niger analytical chromatofocusing, at 23°C 5.9 5.6