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Literature summary extracted from

  • Yamashita, Y.; Ashihara, H.
    Characterization of hexokinase and fructokinase from suspension-cultured Catharanthus roseus cells (1988), Z. Naturforsch. C, 43, 827-834.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP 4 mM: 50% inhibition Catharanthus roseus
2.7.1.1 AMP weak Catharanthus roseus
2.7.1.1 UDP weak Catharanthus roseus
2.7.1.1 UMP very weak Catharanthus roseus
2.7.1.4 ADP
-
 Catharanthus roseus
2.7.1.4 AMP
-
 Catharanthus roseus
2.7.1.4 UDP
-
 Catharanthus roseus
2.7.1.4 UMP
-
 Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.052
-
 ATP
-
 Catharanthus roseus
2.7.1.1 0.06
-
 D-glucose
-
 Catharanthus roseus
2.7.1.1 0.23
-
 D-fructose
-
 Catharanthus roseus
2.7.1.1 0.288
-
 UTP
-
 Catharanthus roseus
2.7.1.4 0.05
-
 ATP with D-fructose as cosubstrate, pH 7.4, 30°C Catharanthus roseus
2.7.1.4 0.13
-
 D-fructose with UTP as cosubstrate, pH 7.4, 30°C Catharanthus roseus
2.7.1.4 0.15
-
 UTP with D-fructose as cosubstrate, pH 7.4, 30°C Catharanthus roseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ Mg2+ or Mn2+ required Catharanthus roseus
2.7.1.1 Mn2+ Mg2+ or Mn2+ required Catharanthus roseus
2.7.1.4 Ca2+ can partially replace Mg2+ or Mn2+ in activation Catharanthus roseus
2.7.1.4 Mg2+ Mg2+ or Mn2+ required Catharanthus roseus
2.7.1.4 Mn2+ Mn2+ or Mg2+ required Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-fructose + ATP Catharanthus roseus fructose metabolism D-fructose 6-phosphate + ADP
-
 ?
2.7.1.1 D-glucose + ATP Catharanthus roseus glucose metabolism D-glucose 6-phosphate + ADP
-
 ?
2.7.1.4 ATP + D-fructose Catharanthus roseus
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Catharanthus roseus
-
 identical with Vinca rosea
-
2.7.1.1 Catharanthus roseus B/TH
-
 identical with Vinca rosea
-
2.7.1.4 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 partial Catharanthus roseus
2.7.1.4 partial Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 cell suspension culture
-
 Catharanthus roseus
-
2.7.1.4 cell culture
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 0.8
-
 at 30°C Catharanthus roseus
2.7.1.4 additional information
-
-
 Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 CTP + D-glucose 35% of the activity with ATP Catharanthus roseus CDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 CTP + D-glucose 35% of the activity with ATP Catharanthus roseus B/TH CDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP
-
 Catharanthus roseus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP
-
 Catharanthus roseus B/TH ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP fructose metabolism Catharanthus roseus D-fructose 6-phosphate + ADP
-
 ?
2.7.1.1 D-glucose + ATP
-
 Catharanthus roseus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Catharanthus roseus B/TH ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP glucose metabolism Catharanthus roseus D-glucose 6-phosphate + ADP
-
 ?
2.7.1.1 GTP + D-glucose 19% of the activity with ATP Catharanthus roseus GDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 GTP + D-glucose 19% of the activity with ATP Catharanthus roseus B/TH GDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 UTP + D-glucose 23% of the activity with ATP Catharanthus roseus UDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 UTP + D-glucose 23% of the activity with ATP Catharanthus roseus B/TH UDP + D-glucose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose
-
 Catharanthus roseus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose specific for fructose Catharanthus roseus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 CTP + D-fructose higher activity than with ATP Catharanthus roseus CDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 additional information no substrate: D-glucose Catharanthus roseus ?
-
 ?
2.7.1.4 UTP + D-fructose higher activity than with ATP Catharanthus roseus UDP + D-fructose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 30
-
 assay at Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8
-
-
 Catharanthus roseus
2.7.1.4 7.2
-
-
 Catharanthus roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.4 6 8.5 pH 6: about 55% of activity maximum, pH 8.5: about 35% of activity maximum Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP requirement Catharanthus roseus