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Literature summary extracted from

  • Fornaini, G.; Dacha, M.; Magnani, M.; Stocchi, V.
    Hexokinase from rabbit red blood cells (1982), Methods Enzymol., 90, 3-10.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.1 D-glucose or D-fructose, 5 mM, stabilizes Oryctolagus cuniculus
2.7.1.1 glycerol, 9-15% v/v, stabilizes Oryctolagus cuniculus
2.7.1.1 sulfhydryl protecting agents stabilize Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ATP uncomplexed, competitive to MgATP2-, erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 D-glucose 6-phosphate linear competitive inhibition with MgATP2- as varied substrate Oryctolagus cuniculus
2.7.1.1 GSSG strong inhibitor at all concentrations Oryctolagus cuniculus
2.7.1.1 Mg2+ competitive to MgATP2-; uncomplexed Oryctolagus cuniculus
2.7.1.1 MgADP- erythrocyte enzyme, mixed inhibitor versus MgATP2- and D-glucose Oryctolagus cuniculus
2.7.1.1 N-acetyl-D-glucosamine competitive to glucose; erythrocyte enzyme; noncompetitive to MgATP2- Oryctolagus cuniculus
2.7.1.1 reduced glutathione 1 mM: reduced state of the enzyme with full catalytic activity, marked inhibition at high concentrations Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.04
-
 D-glucose reticulocyte hexokinase Ia Oryctolagus cuniculus
2.7.1.1 0.06
-
 D-glucose erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 0.125
-
 D-glucose reticulocyte hexokinase Ib Oryctolagus cuniculus
2.7.1.1 0.5
-
 ATP MgATP2- Oryctolagus cuniculus
2.7.1.1 0.5
-
 ATP reticulocyte and erythrocyte enzymes Oryctolagus cuniculus
2.7.1.1 0.6
-
 ATP MgATP2-, erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 0.71
-
 D-mannose erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 1.33
-
 2-deoxy-D-glucose erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 1.9
-
 ITP MgITP2-, erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 2
-
 D-glucosamine erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 17.8
-
 D-fructose erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 41.6
-
 N-acetyl-D-glucosamine erythrocyte enzyme Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 104000
-
 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE Oryctolagus cuniculus
2.7.1.1 105000
-
 reticulocyte hexokinase Ia and Ib, gel filtration, sucrose density gradient sedimentation Oryctolagus cuniculus
2.7.1.1 110000
-
 gel filtration Oryctolagus cuniculus
2.7.1.1 110000
-
 erythrocyte enzyme Oryctolagus cuniculus
2.7.1.1 112000
-
 erythrocyte enzyme, sedimentation velocity Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 erythrocyte enzyme and reticulocyte hexokinase Ia and Ib Oryctolagus cuniculus
2.7.1.1 erythrocyte enzyme: 300000fold Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.1 ATP + D-hexose = ADP + D-hexose 6-phosphate rapid random kinetic mechanism, erythrocyte enzyme and hexokinase I Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 erythrocyte only one enzyme form Oryctolagus cuniculus
-
2.7.1.1 reticulocyte hexokinase Ia and Ib Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 144
-
 reticulocyte, hexokinase Ib, at 37°C Oryctolagus cuniculus
2.7.1.1 145.6
-
 erythrocyte, at 37°C Oryctolagus cuniculus
2.7.1.1 155
-
 reticulocyte, hexokinase Ia, at 37°C Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.1 -20°C, 5 mM sodium-potassium phosphate buffer, pH 7.5, erythrocyte enzyme is more stable than in other buffer systems Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose 65.7% of the activity with glucose, erythrocyte enzyme Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP 70% of the activity with glucose, erythrocyte enzyme Oryctolagus cuniculus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP erythrocyte enzyme, 43.4% of the activity with glucose Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP MgATP2- Oryctolagus cuniculus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 109% of the activity with glucose, erythrocyte enzyme Oryctolagus cuniculus ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose 36% of the activity with ATP, erythrocyte enzyme Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose MgITP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information not: D-galactose Oryctolagus cuniculus ?
-
 ?
2.7.1.1 N-acetyl-D-glucosamine + ATP erythrocyte enzyme, 32.6% of the activity with glucose Oryctolagus cuniculus ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 110000, erythrocyte enzyme, SDS-PAGE Oryctolagus cuniculus
2.7.1.1 monomer 1 * 104000, reticulocyte hexokinase Ia and Ib, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 37
-
 assay at Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.025
-
 D-glucose 6-phosphate
-
 Oryctolagus cuniculus
2.7.1.1 0.7
-
 N-acetyl-D-glucosamine erythrocyte enzyme, competitive to glucose Oryctolagus cuniculus
2.7.1.1 1.5
-
 ATP uncomplexed ATP, erythrocyte enzyme, competitive to MgATP2- Oryctolagus cuniculus
2.7.1.1 3
-
 MgADP- erythrocyte enzyme, mixed inhibitor versus MgATP2- Oryctolagus cuniculus
2.7.1.1 7.8
-
 MgADP- erythrocyte enzyme, mixed inhibitor versus glucose Oryctolagus cuniculus
2.7.1.1 9
-
 Mg2+ erythrocyte enzyme, uncomplexed Mg2+ Oryctolagus cuniculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Oryctolagus cuniculus under native conditions
-
 5.7
2.7.1.1 Oryctolagus cuniculus reticulocyte hexokinase Ib
-
 5.7
2.7.1.1 Oryctolagus cuniculus erythrocyte enzyme and reticulocyte hexokinase Ia, under native conditions
-
 6.2