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Literature summary extracted from

  • Van Leuven, F.
    Highly purified glutamine transaminase from rat brain. Physical and kinetic properties (1975), Eur. J. Biochem., 58, 153-158.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.64 2-oxo acids protect against heat denaturation Rattus norvegicus
2.6.1.64 rather unstable in concentrated ammonium sulfate solution Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.64 hydroxylamine 36 mM, 87% inhibition Rattus norvegicus
2.6.1.64 Isonicotinic acid hydrazide 5 mM, 80% inhibition Rattus norvegicus
2.6.1.64 p-chloromercuribenzoate 0.2 mM, 92% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.64 0.6
-
 L-glutamine pH 9.0, 37.5°C Rattus norvegicus
2.6.1.64 1.5
-
 glyoxylate pH 9.0, 37.5°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.64 50000
-
 2 * 50000, SDS-PAGE Rattus norvegicus
2.6.1.64 98000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.64 L-glutamine + phenylpyruvate Rattus norvegicus
-
 2-oxoglutaramate + L-phenylalanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.64 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.64
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.64 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.64 2.65
-
 brain enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.64 L-glutamine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoglutaramate + L-glutamate
-
 r
2.6.1.64 L-glutamine + glyoxylate
-
 Rattus norvegicus 2-oxoglutaramate + glycine
-
 r
2.6.1.64 L-glutamine + oxaloacetate
-
 Rattus norvegicus 2-oxoglutaramate + L-aspartate
-
 r
2.6.1.64 L-glutamine + phenylpyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-phenylalanine
-
 r
2.6.1.64 L-glutamine + pyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-alanine
-
 r
2.6.1.64 additional information no transamination detected with glutamate, aspartate, asparagine, glycine, alanine, 4-aminobutyrate or cysteine as substrate Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.64 dimer 2 * 50000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.64 60
-
 stable in presence of pyruvate, 90% activity recovered after 30 min, all activity is lost after 15 min in absence of 2-oxoacids Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.64 9 9.2
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.64 8.2 10 about half-maximal activity at pH 8.2 and 10.0 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.64 pyridoxal 5'-phosphate
-
 Rattus norvegicus