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Literature summary extracted from

  • Lucher, L.A.; Chen, Y.M.; Walker, J.B.
    Reactions catalyzed by purified L-glutamine:keto-scyllo-inositol aminotransferase, an enzyme required for biosynthesis of aminocyclitol antibiotics (1989), Antimicrob. Agents Chemother., 33, 452-459.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.6.1.50 pharmacology 2-deoxystreptamine is a component of numerous clinically important antibiotics such as gentamicin, neomycin, tobramycin, amikacin and hygromycin Micromonospora echinospora

General Stability

EC Number General Stability Organism
2.6.1.50 activity rapidly lost when dialyzed Micromonospora echinospora

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.50 5,5'-dithio-bis(2-nitrobenzoic acid) reduces specific activity by 50% Micromonospora echinospora
2.6.1.50 p-hydroxymercuribenzoate reduces specific activity by 50% Micromonospora echinospora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.50 64000 76000 gel filtration Micromonospora echinospora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone Micromonospora echinospora 2-deoxystreptamine biosynthesis 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.50 Micromonospora echinospora
-
 ATCC 15835
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.50
-
 Micromonospora echinospora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.50 mycelium
-
 Micromonospora echinospora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.50 0.24
-
-
 Micromonospora echinospora

Storage Stability

EC Number Storage Stability Organism
2.6.1.50 -20°C, stored at concentrations greater than 1mg/ml, stable for several months with little loss of activity Micromonospora echinospora
2.6.1.50 4°C, inactivated within 1 month Micromonospora echinospora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.50 2-deoxystreptamine + 2-ketoglutaramate
-
 Micromonospora echinospora 2-deoxy-keto-inosamine + L-glutamine
-
 r
2.6.1.50 2-deoxystreptamine + 2-oxoglutaramate
-
 Micromonospora echinospora 2-deoxy-scyllo-inositol + L-glutamine
-
 r
2.6.1.50 2-deoxystreptamine + keto-scyllo-inositol
-
 Micromonospora echinospora 2-deoxy-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
-
 r
2.6.1.50 aminodeoxy-scyllo-inositol + 2-oxoglutaramate
-
 Micromonospora echinospora scyllo-inositol + L-glutamine
-
 r
2.6.1.50 aminodeoxy-scyllo-inositol + keto-scyllo-inositol
-
 Micromonospora echinospora amino-scyllo-inositol + aminodeoxy-keto-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone keto-scyllo-inositol Micromonospora echinospora 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone 2-deoxystreptamine biosynthesis Micromonospora echinospora 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 L-glutamine + 2-oxoadipate
-
 Micromonospora echinospora 2-oxoglutaramate + L-2-amino-1,6-hexandioate
-
 r
2.6.1.50 L-glutamine + 2-oxobutyrate
-
 Micromonospora echinospora 2-oxoglutaramate + 2-aminobutyrate
-
 r
2.6.1.50 L-glutamine + 2-oxocaproate
-
 Micromonospora echinospora 2-oxoglutaramate + L-norleucine
-
 r
2.6.1.50 L-glutamine + 2-oxoglutaramate
-
 Micromonospora echinospora 2-oxoglutaramate + L-glutamine
-
 r
2.6.1.50 L-glutamine + 2-oxoglutarate
-
 Micromonospora echinospora 2-oxoglutaramate + L-glutamate
-
 r
2.6.1.50 L-glutamine + 2-oxovalerate
-
 Micromonospora echinospora 2-oxoglutaramate + L-norvaline
-
 r
2.6.1.50 L-glutamine + DL-epi-inosose
-
 Micromonospora echinospora 2-oxoglutaramate + amino-deoxy-inosose
-
 r
2.6.1.50 L-glutamine + glyoxalate
-
 Micromonospora echinospora 2-oxoglutaramate + glycine
-
 r
2.6.1.50 L-glutamine + oxaloacetate
-
 Micromonospora echinospora 2-oxoglutaramate + L-aspartate
-
 r
2.6.1.50 L-glutamine + pyruvate
-
 Micromonospora echinospora 2-oxoglutaramate + L-alanine
-
 r
2.6.1.50 N3-methyl-2-deoxy-streptamine + keto-scyllo-inositol
-
 Micromonospora echinospora 3-methylamino-2-deoxy-scyllo-inositol + 1-amino-1-deoxy-scyllo-inositol
-
 r
2.6.1.50 N3-methyl-2-deoxystreptamine + 2-oxoglutaramate
-
 Micromonospora echinospora 3-methylamino-2-deoxy-scyllo-inositol + L-glutamine
-
 r
2.6.1.50 streptamine + 2-oxoglutaramate
-
 Micromonospora echinospora amino-scyllo-inositol + L-glutamine
-
 r
2.6.1.50 streptamine + keto-scyllo-inositol
-
 Micromonospora echinospora amino-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.50 aminocyclitol aminotransferase
-
 Micromonospora echinospora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.50 50
-
 heat labile, loses all activity upon incubation for 20 min Micromonospora echinospora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.50 7.5 8.5
-
 Micromonospora echinospora

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.50 7.5 9
-
 Micromonospora echinospora

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.50 pyridoxal 5'-phosphate
-
 Micromonospora echinospora