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Literature summary extracted from

  • Han, Q.; Li, J.
    Comparative characterization of Aedes 3-hydroxykynurenine transaminase/alanine glyoxylate transaminase and Drosophila serine pyruvate aminotransferase (2002), FEBS Lett., 527, 199-204.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.44
-
 Drosophila melanogaster
2.6.1.44 cDNA isolation Aedes aegypti
2.6.1.51 expressed in SF9 cells, using a insect/baculovirus expression system Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.44 1.6
-
 glyoxylate pH 7.0, 50°C, alanine as amino donor Aedes aegypti
2.6.1.44 18
-
 L-alanine pH 7.0, 50°C, glyoxylate as amino acceptor Aedes aegypti
2.6.1.44 51
-
 pyruvate pH 7.0, 50°C, serine as amino donor Drosophila melanogaster
2.6.1.44 64
-
 glyoxylate pH 7.0, 50°C, alanine as amino donor Drosophila melanogaster
2.6.1.44 149
-
 L-alanine pH 7.0, 50°C, glyoxylate as amino acceptor Drosophila melanogaster
2.6.1.44 256
-
 L-serine pH 7.0, 50°C, glyoxylate as amino acceptor Drosophila melanogaster
2.6.1.51 51
-
 pyruvate pH 7.0, 50°C, recombinant enzyme Drosophila melanogaster
2.6.1.51 64
-
 glyoxylate pH 7.0, 50°C, recombinant enzyme Drosophila melanogaster
2.6.1.51 149
-
 alanine pH 7.0, 50°C, recombinant enzyme Drosophila melanogaster
2.6.1.51 256
-
 serine pH 7.0, 50°C, recombinant enzyme Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.44 L-alanine + glyoxylate Aedes aegypti
-
 pyruvate + glycine
-
 ir
2.6.1.44 L-alanine + glyoxylate Drosophila melanogaster
-
 pyruvate + glycine
-
 ir
2.6.1.51 L-serine + pyruvate Drosophila melanogaster glyoxylate detoxification, physiological role in tryptophan catabolism 3-hydroxypyruvate + L-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.44 Aedes aegypti
-
-
-
2.6.1.44 Drosophila melanogaster Q9W3Z3 fruit fly
-
2.6.1.51 Drosophila melanogaster Q9W3Z3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.51 recombinant enzyme Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.44 glycine + pyruvate
-
 Aedes aegypti glyoxylate + L-alanine
-
 ir
2.6.1.44 glycine + pyruvate
-
 Drosophila melanogaster glyoxylate + L-alanine
-
 ir
2.6.1.44 L-2-aminobutyrate + glyoxylate
-
 Aedes aegypti 2-oxobutanoate + glycine
-
 ir
2.6.1.44 L-2-aminobutyrate + glyoxylate
-
 Drosophila melanogaster 2-oxobutanoate + glycine
-
 ir
2.6.1.44 L-alanine + glyoxylate
-
 Aedes aegypti pyruvate + glycine
-
 ir
2.6.1.44 L-alanine + glyoxylate
-
 Drosophila melanogaster pyruvate + glycine
-
 ir
2.6.1.44 L-arginine + pyruvate
-
 Aedes aegypti 5-guanidino-2-oxopentanoate + L-alanine
-
 ir
2.6.1.44 L-arginine + pyruvate
-
 Drosophila melanogaster 5-guanidino-2-oxopentanoate + L-alanine
-
 ir
2.6.1.44 L-cysteine + pyruvate
-
 Aedes aegypti 3-mercapto-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-cysteine + pyruvate
-
 Drosophila melanogaster 3-mercapto-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-glutamine + glyoxylate
-
 Aedes aegypti 2-oxoglutaramate + glycine
-
 ir
2.6.1.44 L-glutamine + pyruvate
-
 Aedes aegypti 2-oxoglutaramate + L-alanine
-
 ir
2.6.1.44 L-histidine + glyoxylate
-
 Aedes aegypti 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-histidine + glyoxylate
-
 Drosophila melanogaster 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-histidine + pyruvate
-
 Drosophila melanogaster 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-methionine + glyoxylate
-
 Aedes aegypti 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ir
2.6.1.44 L-methionine + glyoxylate
-
 Drosophila melanogaster 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ir
2.6.1.44 L-methionine + pyruvate
-
 Aedes aegypti 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
 ir
2.6.1.44 L-methionine + pyruvate
-
 Drosophila melanogaster 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
 ir
2.6.1.44 L-phenylalanine + glyoxylate
-
 Aedes aegypti phenylpyruvate + glycine
-
 ir
2.6.1.44 L-phenylalanine + glyoxylate
-
 Drosophila melanogaster phenylpyruvate + glycine
-
 ir
2.6.1.44 L-phenylalanine + pyruvate
-
 Aedes aegypti phenylpyruvate + L-alanine
-
 ir
2.6.1.44 L-phenylalanine + pyruvate
-
 Drosophila melanogaster phenylpyruvate + L-alanine
-
 ir
2.6.1.44 L-serine + glyoxylate
-
 Aedes aegypti 3-hydroxy-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-serine + glyoxylate
-
 Drosophila melanogaster 3-hydroxy-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-serine + pyruvate
-
 Aedes aegypti 3-hydroxy-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-serine + pyruvate
-
 Drosophila melanogaster 3-hydroxy-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-tryptophan + glyoxylate
-
 Aedes aegypti 3-indole-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-tryptophan + glyoxylate
-
 Drosophila melanogaster 3-indole-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-tryptophan + pyruvate
-
 Aedes aegypti 3-indole-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-tryptophan + pyruvate
-
 Drosophila melanogaster 3-indole-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 L-tyrosine + glyoxylate
-
 Drosophila melanogaster 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
 ir
2.6.1.44 L-tyrosine + pyruvate
-
 Drosophila melanogaster 3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
-
 ir
2.6.1.44 additional information aminoadipate, asparagine, aspartate, glutamate, isoleucine, lysine, threonine and valine are no substrates Aedes aegypti ?
-
 ?
2.6.1.44 additional information aminoadipate, asparagine, aspartate, glutamate, isoleucine, lysine, threonine and valine are no substrates Drosophila melanogaster ?
-
 ?
2.6.1.51 2-aminobutyrate + glyoxylate
-
 Drosophila melanogaster 2-oxobutanoate + glycine
-
 ?
2.6.1.51 arginine + pyruvate
-
 Drosophila melanogaster 5-guanidino-2-oxopentanoate + L-alanine
-
 ?
2.6.1.51 cysteine + pyruvate
-
 Drosophila melanogaster 3-mercapto-2-oxo-propanoate + L-alanine
-
 ?
2.6.1.51 glycine + pyruvate
-
 Drosophila melanogaster glyoxylate + L-alanine
-
 ?
2.6.1.51 histidine + glyoxylate
-
 Drosophila melanogaster 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
 ?
2.6.1.51 histidine + pyruvate
-
 Drosophila melanogaster 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.51 L-alanine + glyoxylate
-
 Drosophila melanogaster pyruvate + glycine
-
 ?
2.6.1.51 L-leucine + pyruvate
-
 Drosophila melanogaster 4-methyl-2-oxopentanoate + L-alanine
-
 ?
2.6.1.51 L-methionine + glyoxylate
-
 Drosophila melanogaster 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ?
2.6.1.51 L-methionine + pyruvate
-
 Drosophila melanogaster 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
 ?
2.6.1.51 L-phenylalanine + glyoxylate
-
 Drosophila melanogaster phenylpyruvate + glycine
-
 ?
2.6.1.51 L-phenylalanine + pyruvate
-
 Drosophila melanogaster phenylpyruvate + L-alanine
-
 ?
2.6.1.51 L-serine + glyoxylate
-
 Drosophila melanogaster glycine + 3-hydroxypyruvate
-
 r
2.6.1.51 L-serine + pyruvate
-
 Drosophila melanogaster 3-hydroxypyruvate + L-alanine
-
 r
2.6.1.51 L-serine + pyruvate glyoxylate detoxification, physiological role in tryptophan catabolism Drosophila melanogaster 3-hydroxypyruvate + L-alanine
-
 r
2.6.1.51 L-tryptophan + glyoxylate
-
 Drosophila melanogaster 3-indole-2-oxopropanoate + glycine
-
 ?
2.6.1.51 L-tyrosine + glyoxylate
-
 Drosophila melanogaster 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
 ?
2.6.1.51 L-tyrosine + pyruvate
-
 Drosophila melanogaster 3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.51 additional information amino-adipate, asparagine, aspartate, glutamate, isoleucine, lysine, threonine and valine are no substrates Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.44 3-hydroxykynurenine transaminase/alanine glyoxylate transaminase
-
 Aedes aegypti
2.6.1.44 serine pyruvate aminotransferase
-
 Drosophila melanogaster
2.6.1.51 Dm-Spat
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.44 30 80 alanine as substrate Drosophila melanogaster
2.6.1.44 60
-
 alanine as substrate Aedes aegypti

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.44 20 80
-
 Aedes aegypti
2.6.1.44 20 80
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.44 16
-
 glyoxylate pH 7.0, 50°C, L-alanine as amino donor Aedes aegypti
2.6.1.44 20
-
 L-alanine pH 7.0, 50°C, glyoxylate as amino acceptor Aedes aegypti
2.6.1.44 113
-
 glyoxylate pH 7.0, 50°C, L-alanine as amino donor Drosophila melanogaster
2.6.1.44 131
-
 L-alanine pH 7.0, 50°C, glyoxylate as amino acceptor Drosophila melanogaster
2.6.1.51 113
-
 glyoxylate
-
 Drosophila melanogaster
2.6.1.51 131
-
 alanine
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.44 7 10
-
 Aedes aegypti
2.6.1.44 7 10
-
 Drosophila melanogaster
2.6.1.44 9
-
 best pH for activity towards alanine Aedes aegypti
2.6.1.44 9
-
 best pH for activity towards alanine Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.44 6 10
-
 Aedes aegypti
2.6.1.44 6 10
-
 Drosophila melanogaster