Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shanker, J.; Datta, K.
    Affinity purification and properties of rat liver mitochondrial L-alanine:4,5-dioxovalerate transaminase and its inhibition by hemin (1986), Arch. Biochem. Biophys., 248, 652-657.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.43 purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.43 2-oxoglutarate
-
 Rattus norvegicus
2.6.1.43 4,5-Dioxopentanoate
-
 Rattus norvegicus
2.6.1.43 hemin uncompetitive inhibitor Rattus norvegicus
2.6.1.43 iodoacetate
-
 Rattus norvegicus
2.6.1.43 methylglyoxal
-
 Rattus norvegicus
2.6.1.43 p-chloromercuric benzene sulfonate
-
 Rattus norvegicus
2.6.1.43 pyruvate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.43 0.28
-
 4,5-Dioxopentanoate pH 6.9, 37°C Rattus norvegicus
2.6.1.43 3.3
-
 L-alanine pH 6.9, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.43 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.43 41000
-
 6 * 41000 Rattus norvegicus
2.6.1.43 232000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate Rattus norvegicus
-
 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate Rattus norvegicus Wistar
-
 5-aminolevulinate + pyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.43 Rattus norvegicus
-
 Wistar
-
2.6.1.43 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.43
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.43 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.43 1.171
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.43 -20°C, remains stable for at least 1 month Rattus norvegicus
2.6.1.43 -70°C, stable for at least 2-3 months Rattus norvegicus
2.6.1.43 4°C, can be stored for at least 1 week without any significant loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Rattus norvegicus 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Rattus norvegicus Wistar 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 additional information DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine Rattus norvegicus ?
-
 ?
2.6.1.43 additional information DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine Rattus norvegicus Wistar ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.43 hexamer 6 * 41000 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.43 pyridoxal 5'-phosphate
-
 Rattus norvegicus