Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shioi, Y.; Nagamine, M.; Sasa, T.
    Purification and properties of L-alanine:4,5-dioxovalerate aminotransferase from Chlorella regularis (1984), Arch. Biochem. Biophys., 234, 117-124.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.43 stability is improved by 10-15% glycerol Chlorella regularis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.43 2-oxoglutarate
-
 Chlorella regularis
2.6.1.43 4,5-Dioxopentanoate substrate inhibition Chlorella regularis
2.6.1.43 amino-oxyacetic acid
-
 Chlorella regularis
2.6.1.43 glyoxylic acid
-
 Chlorella regularis
2.6.1.43 imidazole
-
 Chlorella regularis
2.6.1.43 pyruvate
-
 Chlorella regularis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.43 0.12
-
 4,5-Dioxopentanoate pH 8.0, 37°C Chlorella regularis
2.6.1.43 1.3
-
 glyoxylate pH 8.0, 37°C Chlorella regularis
2.6.1.43 3
-
 L-alanine pH 8.0, 37°C, glyoxylate as amino acceptor Chlorella regularis
2.6.1.43 3.5
-
 L-alanine pH 8.0, 37°C Chlorella regularis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.43 68000
-
 2 * 68000, SDS-PAGE Chlorella regularis
2.6.1.43 126000
-
 gel filtration Chlorella regularis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate Chlorella regularis
-
 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate Chlorella regularis S-50
-
 5-aminolevulinate + pyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.43 Chlorella regularis
-
 S-50
-
2.6.1.43 Chlorella regularis S-50
-
 S-50
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.43
-
 Chlorella regularis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.43 2.68
-
-
 Chlorella regularis

Storage Stability

EC Number Storage Stability Organism
2.6.1.43 4°C, 10-15% glycerol, 20% loss of activity after 5 days Chlorella regularis
2.6.1.43 4°C, unstable Chlorella regularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.43 glycine + 4,5-dioxopentanoate 21% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + glyoxylate
-
 ir
2.6.1.43 glycine + 4,5-dioxopentanoate 21% of the activity with L-alanine Chlorella regularis S-50 5-aminolevulinate + glyoxylate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Chlorella regularis 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Chlorella regularis S-50 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-glutamate + 4,5-dioxopentanoate 37% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 2-oxoglutarate
-
 ir
2.6.1.43 L-glutamate + 4,5-dioxopentanoate 37% of the activity with L-alanine Chlorella regularis S-50 5-aminolevulinate + 2-oxoglutarate
-
 ir
2.6.1.43 L-glutamine + 4,5-dioxopentanoate 16% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 2-oxoglutaramate
-
 ?
2.6.1.43 L-lysine + 4,5-dioxopentanoate 15% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 6-amino-2-oxohexanoate
-
 ir
2.6.1.43 L-serine + 4,5-dioxopentanoate 33% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
 ir
2.6.1.43 L-serine + 4,5-dioxopentanoate 33% of the activity with L-alanine Chlorella regularis S-50 5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
 ir
2.6.1.43 additional information does not use 2-oxoglutarate as amino acceptor Chlorella regularis ?
-
 ?
2.6.1.43 additional information does not use 2-oxoglutarate as amino acceptor Chlorella regularis S-50 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.43 dimer 2 * 68000, SDS-PAGE Chlorella regularis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.43 EC 2.6.1.44 identical with alanine-glyoxylate aminotransferase Chlorella regularis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.43 55
-
 retains 75% activity after 1 h incubation Chlorella regularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.43 8
-
-
 Chlorella regularis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.43 0.5
-
 glyoxylate pH 8.0, 37°C Chlorella regularis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.43 Chlorella regularis isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4°C for 45 h
-
 4.8