Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Neuberger, A.; Turner, J.M.
    gamma, delta-Dioxovalerate aminotransferase activity in Rhodopseudomonas spheroides (1963), Biochim. Biophys. Acta, 67, 342-345.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.43 glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.43 4,5-Dioxopentanoate substrate inhibition Cereibacter sphaeroides
2.6.1.43 acetyl acetone
-
 Cereibacter sphaeroides
2.6.1.43 amino-oxyacetic acid
-
 Cereibacter sphaeroides
2.6.1.43 benzoyl acetone
-
 Cereibacter sphaeroides
2.6.1.43 beta-Alanine 8% inhibition Cereibacter sphaeroides
2.6.1.43 beta-Iodopropionate 30% inhibition Cereibacter sphaeroides
2.6.1.43 cadaverine 14% inhibition Cereibacter sphaeroides
2.6.1.43 cysteamine
-
 Cereibacter sphaeroides
2.6.1.43 cysteine 90% inhibition Cereibacter sphaeroides
2.6.1.43 diacetyl
-
 Cereibacter sphaeroides
2.6.1.43 ethyl acetoacetate
-
 Cereibacter sphaeroides
2.6.1.43 glyoxylic acid
-
 Cereibacter sphaeroides
2.6.1.43 homocysteine
-
 Cereibacter sphaeroides
2.6.1.43 iodoacetate 30% inhibition Cereibacter sphaeroides
2.6.1.43 Isonicotinic acid hydrazide
-
 Cereibacter sphaeroides
2.6.1.43 lysine 20% inhibition Cereibacter sphaeroides
2.6.1.43 additional information iodoacetamide and chloroacetamide are not inhibitors Cereibacter sphaeroides
2.6.1.43 ophthalaldehyde
-
 Cereibacter sphaeroides
2.6.1.43 ornithine 20% inhibition Cereibacter sphaeroides
2.6.1.43 oxaloacetic acid
-
 Cereibacter sphaeroides
2.6.1.43 p-chloromercuribenzoate 78% inhibition Cereibacter sphaeroides
2.6.1.43 Penicillamine
-
 Cereibacter sphaeroides
2.6.1.43 Pyruvic acid
-
 Cereibacter sphaeroides
2.6.1.43 succinic acid
-
 Cereibacter sphaeroides
2.6.1.43 Thioglycollate 74% inhibition Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.43 0.4
-
 4,5-Dioxopentanoate pH 7.0, 37°C Cereibacter sphaeroides
2.6.1.43 8
-
 L-alanine pH 7.0, 37°C Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate Cereibacter sphaeroides
-
 5-aminolevulinate + pyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.43 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.43 partial Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
2.6.1.43 -15°C, stable for several months Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.43 delta-amino-n-valerate + 4,5-dioxopentanoate transaminate at 95% of the rate of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + 5-oxo-n-pentanoate
-
 ir
2.6.1.43 epsilon-amino-n-caproate + 4,5-dioxopentanoate transaminate at 103% of the rate of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + 6-oxo-n-hexanoate
-
 ir
2.6.1.43 gamma-amino-n-butyrate + 4,5-dioxopentanoate transaminate at 122% of the rate of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + succinic semialdehyde
-
 ir
2.6.1.43 glycine + 4,5-dioxopentanoate less than 10% of the activity of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + glyoxylate
-
 ir
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Cereibacter sphaeroides 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-ornithine + 4,5-dioxopentanoate less than 10% of the activity of alpha-alanine Cereibacter sphaeroides 5-aminolevulinate + 5-amino-2-oxopentanoate
-
 ir
2.6.1.43 additional information ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity Cereibacter sphaeroides ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.43 7
-
-
 Cereibacter sphaeroides