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Literature summary extracted from

  • Nakada, H.I.
    Glutamic-glycine transaminase from rat liver (1964), J. Biol. Chem., 239, 468-471.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.4 hydroxylamine 50% inhibition at 5 mM Rattus norvegicus
2.6.1.4 Isonicotinic acid hydrazide glyoxylate partially protects Rattus norvegicus
2.6.1.4 Maleic acid weak Rattus norvegicus
2.6.1.4 PCMB no inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.4 4.6
-
 glutamate pH 7.3 Rattus norvegicus
2.6.1.4 8.3
-
 glyoxylate pH 7.3 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.4 glyoxylate + L-glutamate Rattus norvegicus
-
 glycine + 2-oxoglutarate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.4 partial, about 30fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.4 heart limited activity Rattus norvegicus
-
2.6.1.4 kidney
-
 Rattus norvegicus
-
2.6.1.4 liver
-
 Rattus norvegicus
-
2.6.1.4 additional information tissue distribution, very low activity in lung, testes or diaphragm Rattus norvegicus
-
2.6.1.4 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.4 0.13
-
 partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.4 glyoxylate + L-alanine no activity Rattus norvegicus glycine + pyruvate
-
 ?
2.6.1.4 glyoxylate + L-glutamate
-
 Rattus norvegicus glycine + 2-oxoglutarate
-
 ir
2.6.1.4 additional information substrate specificity Rattus norvegicus ?
-
 ?
2.6.1.4 additional information no substrates are D-amino acid isomers Rattus norvegicus ?
-
 ?
2.6.1.4 additional information no activity with ammonia, glutathione, L-ornithine or 4-aminobutanoate Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.4 glutamic-glycine transaminase
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.4 60
-
 5 min, stable Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.4 7.3
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.4 6.2 8.2 about half-maximal activity at pH 6.2 and pH 8.2 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.4 pyridoxal 5'-phosphate addition to enzyme system mostly without effect Rattus norvegicus