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Literature summary extracted from

  • Wu, H.L.C.; Mason, M.
    Pyridoxamine-oxaloacetic transaminase of rat kidney (1964), J. Biol. Chem., 239, 1492-1497.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.31 arsenate activation, less effective than phosphate Rattus norvegicus
2.6.1.31 D-Aspartate increases activity Rattus norvegicus
2.6.1.31 diphosphate activation, less effective than phosphate Rattus norvegicus
2.6.1.31 phosphate essential, activation in absence of diethylstilbestrol and pyridoxal 5'-phosphate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.31 2-oxoglutarate inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate Rattus norvegicus
2.6.1.31 2-Oxomalonate inhibition at pH 7.0 is higher than at pH 8.5, 0.4 mM in presence of equimolar concentrations of oxaloacetate Rattus norvegicus
2.6.1.31 3,3-Dimethylglutamic acid not at pH 7.0, weak at pH 8.0 Rattus norvegicus
2.6.1.31 3-oxoadipate inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate Rattus norvegicus
2.6.1.31 adipic acid not at pH 7.0, weak at pH 8.0 Rattus norvegicus
2.6.1.31 Benzylmalonic acid not at pH 7.0, weak at pH 8.0 Rattus norvegicus
2.6.1.31 Diethylstilbestrol diphosphate
-
 Rattus norvegicus
2.6.1.31 L-aspartate
-
 Rattus norvegicus
2.6.1.31 L-glutamate
-
 Rattus norvegicus
2.6.1.31 pyridoxal 5'-phosphate
-
 Rattus norvegicus
2.6.1.31 pyridoxamine phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.31 0.012
-
 oxaloacetate somewhat higher at very high levels of pyridoxamine, 14 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.31 pyridoxamine + oxaloacetate Rattus norvegicus
-
 pyridoxal + L-aspartate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.31 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.31 979fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.31 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.31 0.066
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.31 frozen, 20% loss of activity after 2 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.31 pyridoxamine + oxaloacetate
-
 Rattus norvegicus pyridoxal + L-aspartate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.31 8.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.31 7.5 9.5 about 50% of activity maximum at pH 7.5 and pH 9.5 Rattus norvegicus