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Literature summary extracted from

  • Wada, H.; Snell, E.E.
    Enzymatic transamination pf pyridoxamine. II. Crystalline pyridoxamine-pyruvate transaminase (1962), J. Biol. Chem., 237, 133-137.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.30 additional information phosphate is neither essential nor inhibitory Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.30 addition of saturated ammonium sulfate solution to the concentrated protein solution until the first permanent turbidity appears, several days at 5°C Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.30 additional information phosphate is neither essential nor inhibitory Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.30 0.043
-
 pyridoxamine pH 8.5, 37°C Pseudomonas sp.
2.6.1.30 0.1
-
 pyruvate pH 8.5, 37°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.30 pyridoxamine + pyruvate Pseudomonas sp. oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor pyridoxal + L-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.30 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.30
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.30 7.3
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.30 pyridoxamine + pyruvate oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor Pseudomonas sp. pyridoxal + L-alanine
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.30 37
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.30 70
-
 10 min, stable Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.30 additional information
-
 additional information
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.30 8.5
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.30 additional information pyridoxal 5'-phosphate is not required for activity Pseudomonas sp.