EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.64 | 0.1 | - |
2-Oxoglutaramate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
2.6.1.64 | 0.58 | - |
L-phenylalanine | pH 8.5, 37°C | Rattus norvegicus | |
2.6.1.64 | 0.92 | - |
alpha-keto-gamma-methiolbutyrate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
2.6.1.64 | 1.4 | - |
L-glutamine | pH 8.5, 37°C, phenylpyruvate | Rattus norvegicus | |
2.6.1.64 | 4.2 | - |
L-methionine | pH 8.5, 37°C, phenylpyruvate | Rattus norvegicus | |
2.6.1.64 | 4.4 | - |
L-ethionine | pH 8.5, 37°C, phenylpyruvate | Rattus norvegicus | |
2.6.1.64 | 4.8 | - |
glyoxylate | pH 8.5, 37°C | Rattus norvegicus | |
2.6.1.64 | 7.8 | - |
alpha-keto-n-valerate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
2.6.1.64 | 8.7 | - |
alpha-keto-n-caproate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
2.6.1.64 | 18 | - |
alpha-Ketobutyrate | pH 8.5, 37°C | Rattus norvegicus | |
2.6.1.64 | 27 | - |
pyruvate | pH 8.5, 37°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.15 | cytosol | - |
Rattus norvegicus | 5829 | - |
2.6.1.64 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.64 | 55000 | - |
2 * 55000, liver enzyme | Rattus norvegicus |
2.6.1.64 | 110000 | - |
kidney enzyme, gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.15 | L-glutamine + alpha-keto acid | Rattus norvegicus | - |
alpha-ketoglutarate + amino acid | - |
? | |
2.6.1.15 | L-glutamine + alpha-keto acid | Rattus norvegicus Sprague-Dawley | - |
alpha-ketoglutarate + amino acid | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.15 | Rattus norvegicus | - |
male Sprague-Dawley | - |
2.6.1.64 | Rattus norvegicus | - |
Sprague-Dawley | - |
2.6.1.64 | Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.15 | DE-52 chromatography, hydroxyapatite chromatography | Rattus norvegicus |
2.6.1.64 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.15 | liver | - |
Rattus norvegicus | - |
2.6.1.64 | brain | - |
Rattus norvegicus | - |
2.6.1.64 | cardiac muscle | - |
Rattus norvegicus | - |
2.6.1.64 | kidney | - |
Rattus norvegicus | - |
2.6.1.64 | liver | - |
Rattus norvegicus | - |
2.6.1.64 | additional information | no glutamine transaminase activity in skeletal muscle, testis, spleen, pancreas, lung and intestine | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.15 | 0.467 | - |
- |
Rattus norvegicus |
2.6.1.64 | 3.68 | - |
cytoplasmic isozyme | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.15 | 2-oxoglutaramate + L-methionine | - |
Rattus norvegicus | L-glutamine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
2.6.1.15 | gamma-glutamylhydrazones of 2-oxo acid | - |
Rattus norvegicus | L-amino acid + 2-hydroxy-tetrahydro-6-pyridazinone-3-carboxylic acid | - |
? | |
2.6.1.15 | glyoxylate + 4-glutamylmethylamide | - |
Rattus norvegicus | glycine + ? | - |
? | |
2.6.1.15 | glyoxylate + glutamic acid 4-methyl ester | - |
Rattus norvegicus | glycine + 2-oxoglutarate 4-methyl ester | - |
? | |
2.6.1.15 | glyoxylate + L-cysteine | - |
Rattus norvegicus | glycine + 3-mercapto-2-oxopropanoate | - |
? | |
2.6.1.15 | glyoxylate + L-ethionine | - |
Rattus norvegicus | glycine + 4-ethylsulfanyl-2-oxobutanoate | - |
? | |
2.6.1.15 | glyoxylate + L-ethionine | - |
Rattus norvegicus Sprague-Dawley | glycine + 4-ethylsulfanyl-2-oxobutanoate | - |
? | |
2.6.1.15 | glyoxylate + L-glutamine | - |
Rattus norvegicus | glycine + 2-oxoglutaramate | - |
? | |
2.6.1.15 | glyoxylate + L-glutamine | - |
Rattus norvegicus Sprague-Dawley | glycine + 2-oxoglutaramate | - |
? | |
2.6.1.15 | glyoxylate + L-homoserine | - |
Rattus norvegicus | glycine + 4-hydroxy-2-oxobutanoate | - |
? | |
2.6.1.15 | glyoxylate + L-methionine | - |
Rattus norvegicus | glycine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
2.6.1.15 | glyoxylate + L-methionine | - |
Rattus norvegicus Sprague-Dawley | glycine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
2.6.1.15 | glyoxylate + L-methionine (SR)-sulfoxide | - |
Rattus norvegicus | glycine + ? | - |
? | |
2.6.1.15 | glyoxylate + L-methionine (SR)-sulfoxide | - |
Rattus norvegicus Sprague-Dawley | glycine + ? | - |
? | |
2.6.1.15 | glyoxylate + L-methionine sulfone | - |
Rattus norvegicus | glycine + 4-methylsulfonyl-2-oxobutanoate | - |
? | |
2.6.1.15 | glyoxylate + L-phenylalanine | - |
Rattus norvegicus | glycine + phenylpyruvate | - |
? | |
2.6.1.15 | glyoxylate + S-methyl-L-cysteine | - |
Rattus norvegicus | glycine + 3-methylsulfanyl-2-oxopropanoate | - |
? | |
2.6.1.15 | L-glutamine + alpha-keto acid | - |
Rattus norvegicus | alpha-ketoglutarate + amino acid | - |
? | |
2.6.1.15 | L-glutamine + alpha-keto acid | - |
Rattus norvegicus Sprague-Dawley | alpha-ketoglutarate + amino acid | - |
? | |
2.6.1.15 | pyruvate + L-glutamine | - |
Rattus norvegicus | L-alanine + 2-oxoglutaramate | - |
? | |
2.6.1.15 | pyruvate + L-phenylalanine | - |
Rattus norvegicus | L-alanine + phenylpyruvate | - |
? | |
2.6.1.64 | 4-cyano-L-alpha-aminobutyrate + glyoxylate | - |
Rattus norvegicus | 4-cyano-2-oxobutanoate + glycine | - |
r | |
2.6.1.64 | 4-cyano-L-alpha-aminobutyrate + phenylpyruvate | - |
Rattus norvegicus | 4-cyano-2-oxobutanoate + L-phenylalanine | - |
r | |
2.6.1.64 | albizziin + glyoxylate | - |
Rattus norvegicus | 3-carbamido-2-oxopropanoate + glycine | - |
r | |
2.6.1.64 | DL-homoserine + glyoxylate | - |
Rattus norvegicus | 4-hydroxy-2-oxobutanoate + glycine | - |
r | |
2.6.1.64 | DL-homoserine + phenylpyruvate | - |
Rattus norvegicus | 4-hydroxy-2-oxobutanoate + phenylalanine | - |
r | |
2.6.1.64 | L-cysteine + glyoxylate | - |
Rattus norvegicus | 3-mercapto-2-oxopropanoate + glycine | - |
r | |
2.6.1.64 | L-cysteine + phenylpyruvate | - |
Rattus norvegicus | 3-mercapto-2-oxopropanoate + L-phenylalanine | - |
r | |
2.6.1.64 | L-ethionine + glyoxylate | - |
Rattus norvegicus | 4-ethylsulfanyl-2-oxobutanoate + glycine | - |
r | |
2.6.1.64 | L-ethionine + phenylpyruvate | - |
Rattus norvegicus | 4-ethylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
r | |
2.6.1.64 | L-gamma-glutamylhydrazone of glyoxylate + glyoxylate | - |
Rattus norvegicus | ? | - |
r | |
2.6.1.64 | L-gamma-glutamylhydrazone of glyoxylate + phenylpyruvate | - |
Rattus norvegicus | ? | - |
r | |
2.6.1.64 | L-gamma-glutamylhydrazone of phenylpyruvate + glyoxylate | - |
Rattus norvegicus | ? | - |
r | |
2.6.1.64 | L-gamma-glutamylhydrazone of phenylpyruvate + phenylpyruvate | - |
Rattus norvegicus | ? | - |
r | |
2.6.1.64 | L-glutamic acid-gamma-methyl ester + glyoxylate | liver enzyme L | Rattus norvegicus | 2-oxo-pentandioate-5-methyl ester + glycine | - |
r | |
2.6.1.64 | L-glutamic acid-gamma-methyl ester + phenylpyruvate | liver enzyme L | Rattus norvegicus | 2-oxo-pentandioate-5-methyl ester + L-phenylalanine | - |
r | |
2.6.1.64 | L-glutamine + 2-oxobutyrate | - |
Rattus norvegicus | 2-oxoglutaramate + 2-aminobutanoate | - |
r | |
2.6.1.64 | L-glutamine + 2-oxoglutaramate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamine | - |
r | |
2.6.1.64 | L-glutamine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamate | - |
r | |
2.6.1.64 | L-glutamine + 2-oxovalerate | - |
Rattus norvegicus | 2-oxoglutaramate + L-norvaline | - |
r | |
2.6.1.64 | L-glutamine + 3-hydroxypyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-serine | - |
r | |
2.6.1.64 | L-glutamine + alpha-keto-delta-carbamidovalerate | - |
Rattus norvegicus | 2-oxoglutaramate + L-arginine | - |
r | |
2.6.1.64 | L-glutamine + alpha-keto-n-caproate | - |
Rattus norvegicus | 2-oxoglutaramate + L-norleucine | - |
r | |
2.6.1.64 | L-glutamine + alpha-ketoisocaproate | - |
Rattus norvegicus | 2-oxoglutaramate + L-leucine | - |
r | |
2.6.1.64 | L-glutamine + alpha-ketoisovalerate | - |
Rattus norvegicus | 2-oxoglutaramate + L-valine | - |
r | |
2.6.1.64 | L-glutamine + glyoxylate | isozyme L highly active, isozyme K relatively little activity toward glyoxylate | Rattus norvegicus | 2-oxoglutaramate + glycine | - |
r | |
2.6.1.64 | L-glutamine + oxaloacetate | - |
Rattus norvegicus | 2-oxoglutaramate + L-aspartate | - |
r | |
2.6.1.64 | L-glutamine + p-hydroxyphenylpyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-tyrosine | - |
r | |
2.6.1.64 | L-glutamine + phenylpyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-phenylalanine | - |
r | |
2.6.1.64 | L-glutamine + pyruvate | isozyme L highly active, isozyme K relatively little activity toward pyruvate | Rattus norvegicus | 2-oxoglutaramate + L-alanine | - |
r | |
2.6.1.64 | L-glutamylmethylamide + glyoxylate | liver enzyme L | Rattus norvegicus | 2-oxoglutarate-5-methylamide + glycine | - |
r | |
2.6.1.64 | L-glutamylmethylamide + phenylpyruvate | - |
Rattus norvegicus | 2-oxoglutarate-5-methylamide + L-phenylalanine | - |
r | |
2.6.1.64 | L-histidine + pyruvate | poor substrates | Rattus norvegicus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
r | |
2.6.1.64 | L-methionine + 2-oxoglutaramate | isozymes L and K highly active | Rattus norvegicus | L-glutamine + 4-methylsulfanyl-2-oxobutanoate | - |
r | |
2.6.1.64 | L-methionine + glyoxylate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + glycine | - |
r | |
2.6.1.64 | L-methionine + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
r | |
2.6.1.64 | L-methionine sulfone + glyoxylate | - |
Rattus norvegicus | 4-methylsulfonyl-2-oxobutanoate + glycine | - |
r | |
2.6.1.64 | L-methionine sulfone + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfonyl-2-oxobutanoate + L-phenylalanine | - |
r | |
2.6.1.64 | L-methionine-SR-sulfoxide + glyoxylate | - |
Rattus norvegicus | 4-methylsulfinyl-2-oxobutanoate + glycine | - |
r | |
2.6.1.64 | L-methionine-SR-sulfoxide + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfinyl-2-oxobutanoate + L-phenylalanine | - |
r | |
2.6.1.64 | L-methionine-SR-sulfoximine + glyoxylate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + glycine | - |
r | |
2.6.1.64 | L-methionine-SR-sulfoximine + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + L-phenylalanine | - |
r | |
2.6.1.64 | L-phenylalanine + 2-oxoglutaramate | - |
Rattus norvegicus | phenylpyruvate + L-glutamine | - |
r | |
2.6.1.64 | L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate | - |
Rattus norvegicus | phenylpyruvate + L-methionine | - |
r | |
2.6.1.64 | L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate | isozyme K very active toward phenylalanine | Rattus norvegicus | phenylpyruvate + L-methionine | - |
r | |
2.6.1.64 | L-phenylalanine + alpha-keto-n-caproate | - |
Rattus norvegicus | phenylpyruvate + L-norleucine | - |
r | |
2.6.1.64 | L-phenylalanine + alpha-keto-n-valerate | - |
Rattus norvegicus | phenylpyruvate + L-norvaline | - |
r | |
2.6.1.64 | L-phenylalanine + alpha-ketobutyrate | - |
Rattus norvegicus | phenylpyruvate + 2-aminobutyrate | - |
r | |
2.6.1.64 | L-phenylalanine + glyoxylate | - |
Rattus norvegicus | phenylpyruvate + glycine | - |
r | |
2.6.1.64 | L-phenylalanine + pyruvate | - |
Rattus norvegicus | phenylpyruvate + L-alanine | - |
r | |
2.6.1.64 | L-tyrosine + 2-oxoglutaramate | - |
Rattus norvegicus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine | - |
r | |
2.6.1.64 | additional information | 4 separate isozymes, cytoplasmic and mitochondrial, in kidney and liver, trimethylpyruvate and D and L-alpha-keto-beta-methylvalerate are no amino acceptors, L-gamma-glutamyldimethylamide is no substrate | Rattus norvegicus | ? | - |
? | |
2.6.1.64 | O-carbamoyl-L-serine + glyoxylate | - |
Rattus norvegicus | O-carbamoyl-(3-hydroxy-2-oxo-propanoate) + glycine | - |
r | |
2.6.1.64 | S-carbamoyl-L-cysteine + glyoxylate | - |
Rattus norvegicus | S-carbamoyl-3-mercapto-2-oxo-propanoate + glycine | - |
r | |
2.6.1.64 | S-methyl-L-cysteine + glyoxylate | - |
Rattus norvegicus | 3-methylsulfanyl-2-oxopropanoate + glycine | - |
r | |
2.6.1.64 | S-methyl-L-cysteine + phenylpyruvate | - |
Rattus norvegicus | 3-methylsulfanyl-2-oxopropanoate + L-phenylalanine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.64 | dimer | 2 * 55000, liver enzyme | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.64 | glutamine transaminase K | - |
Rattus norvegicus |
2.6.1.64 | glutamine transaminase L | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.15 | 37 | - |
assay at | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.64 | 60 | - |
liver glutaminase transaminase L is unstable at 60°C in absence of an added alpha-keto-acid, kidney glutamine transaminase L is stable | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.64 | pyridoxal 5'-phosphate | - |
Rattus norvegicus |