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Literature summary extracted from

  • Cooper, A.J.L.; Meister, A.
    Isolation and properties of highly purified glutamine transaminase (1972), Biochemistry, 11, 661-671.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.15 2-mercaptoethanol and alpha-keto acids stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.15 3-Mercaptopyruvate
-
 Rattus norvegicus
2.6.1.15 4-methylsulfanyl-2-oxobutanoate
-
 Rattus norvegicus
2.6.1.15 carbonyl reagents
-
 Rattus norvegicus
2.6.1.15 hydroxylamine
-
 Rattus norvegicus
2.6.1.15 Isonicotinic acid hydrazide
-
 Rattus norvegicus
2.6.1.15 Sodium borohydride
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.15 1.9
-
 L-methionine pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 2
-
 L-glutamine pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 2
-
 L-methionine (SR)-sulfoxide pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 3.2
-
 4-methylsulfanyl-2-oxobutanoate pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 4.4
-
 L-glutamic acid gamma-methylester pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 4.8
-
 3-Mercaptopyruvate pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 8
-
 glyoxylate pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 8
-
 3-hydroxypyruvate pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 10.5
-
 pyruvate pH 8.4, 37ºC Rattus norvegicus
2.6.1.15 50
-
 2-Oxoglutaramate pH 8.4, 37ºC Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.15 110000
-
 PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.15 L-glutamine + alpha-keto acid Rattus norvegicus
-
 alpha-ketoglutarate + amino acid
-
 ?
2.6.1.15 L-glutamine + pyruvate Rattus norvegicus
-
 2-oxoglutaramate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.15 Rattus norvegicus
-
 male Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.15 ammonium sulfate fractionation , DE-52 chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.15 5
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.15 -20°C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, at least 7 weeks Rattus norvegicus
2.6.1.15 0-5°C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, little or no loss of activity for 1-2 weeks, in the absence of mercaptoethanol inactivation within 1-2 days Rattus norvegicus
2.6.1.15 24-26°C, 20% loss of activity of purified enzyme in 3 days Rattus norvegicus
2.6.1.15 4ºC, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.15 2-oxoglutaramate + glycine poor substrate Rattus norvegicus L-glutamine + glyoxylate
-
 ?
2.6.1.15 2-oxoglutaramate + L-alanine poor substrate Rattus norvegicus L-glutamine + pyruvate
-
 ?
2.6.1.15 2-oxoglutaramate + L-asparagine poor substrate Rattus norvegicus L-glutamine + 2-oxosuccinamate
-
 ?
2.6.1.15 2-oxoglutaramate + L-cysteine poor substrate Rattus norvegicus L-glutamine + 3-mercapto-2-oxopropanoate
-
 ?
2.6.1.15 2-oxoglutaramate + L-ethionine
-
 Rattus norvegicus L-glutamine + 4-ethylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 2-oxoglutaramate + L-glutamic acid 4-benzylester poor substrate Rattus norvegicus L-glutamine + 2-oxopentane-1,5-dioic acid benzyl ester
-
 ?
2.6.1.15 2-oxoglutaramate + L-homoserine
-
 Rattus norvegicus L-glutamine + 4-hydroxy-2-oxobutanoate
-
 ?
2.6.1.15 2-oxoglutaramate + L-methionine best amino group donor Rattus norvegicus L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 2-oxoglutaramate + L-methionine (SR)-sulfoxide
-
 Rattus norvegicus L-glutamine + 4-methylsulfinyl-2-oxobutanoate
-
 ?
2.6.1.15 2-oxoglutaramate + L-methionine sulfone
-
 Rattus norvegicus L-glutamine + 4-methylsulfonyl-2-oxobutanoate
-
 ?
2.6.1.15 2-oxoglutaramate + L-phenylalanine poor substrate Rattus norvegicus L-glutamine + phenylpyruvate
-
 ?
2.6.1.15 2-oxoglutaramate + L-serine poor substrate Rattus norvegicus L-glutamine + 3-hydroxy-2-oxopropanoate
-
 ?
2.6.1.15 glyoxylate + 4-glutamylmethylamide
-
 Rattus norvegicus glycine + ?
-
 ?
2.6.1.15 glyoxylate + DL-homocysteine poor substrate Rattus norvegicus glycine + 2-oxo-4-thiobutanoate
-
 ?
2.6.1.15 glyoxylate + glutamic acid 4-methyl ester
-
 Rattus norvegicus glycine + 2-oxoglutarate 4-methyl ester
-
 ?
2.6.1.15 glyoxylate + L-2-amino-4-oxo-5-chloropentanoic acid poor substrate Rattus norvegicus glycine + 5-chloro-2,4-dioxopentanoate
-
 ?
2.6.1.15 glyoxylate + L-alanine poor substrate Rattus norvegicus glycine + pyruvate
-
 ?
2.6.1.15 glyoxylate + L-asparagine poor substrate Rattus norvegicus glycine + 2-oxosuccinamate
-
 ?
2.6.1.15 glyoxylate + L-cysteine poor substrate Rattus norvegicus glycine + 3-mercapto-2-oxopropanoate
-
 ?
2.6.1.15 glyoxylate + L-ethionine
-
 Rattus norvegicus glycine + 4-ethylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-glutamine
-
 Rattus norvegicus glycine + 2-oxoglutaramate
-
 ?
2.6.1.15 glyoxylate + L-homoserine poor substrate Rattus norvegicus glycine + 4-hydroxy-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-methionine
-
 Rattus norvegicus glycine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-methionine (SR)-sulfoxide
-
 Rattus norvegicus glycine + ?
-
 ?
2.6.1.15 glyoxylate + L-methionine sulfone
-
 Rattus norvegicus glycine + 4-methylsulfonyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-phenylalanine poor substrate Rattus norvegicus glycine + phenylpyruvate
-
 ?
2.6.1.15 glyoxylate + L-serine poor substrate Rattus norvegicus glycine + 3-hydroxy-2-oxopropanoate
-
 ?
2.6.1.15 glyoxylate + S-methyl-L-cysteine poor substrate Rattus norvegicus glycine + 3-methylsulfanyl-2-oxopropanoate
-
 ?
2.6.1.15 L-glutamine + 2-oxoglutaramate
-
 Rattus norvegicus 2-oxoglutaramate + L-glutamine
-
 ?
2.6.1.15 L-glutamine + 3-hydroxypyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-serine
-
 ?
2.6.1.15 L-glutamine + 3-mercaptopyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-cysteine
-
 ?
2.6.1.15 L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 Rattus norvegicus 2-oxoglutaramate + L-methionine
-
 ?
2.6.1.15 L-glutamine + alpha-keto acid
-
 Rattus norvegicus alpha-ketoglutarate + amino acid
-
 ?
2.6.1.15 L-glutamine + pyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-alanine
-
 ?
2.6.1.15 pyruvate + L-4-glutamylmethylamide
-
 Rattus norvegicus L-alanine + ?
-
 ?
2.6.1.15 pyruvate + L-glutamine L-glutamine cannot be replaced by glycine Rattus norvegicus L-alanine + 2-oxoglutaramate
-
 ?
2.6.1.15 pyruvate + L-methionine poor substrate Rattus norvegicus L-alanine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 pyruvate + L-methionine (SR)-sulfoxide poor substrate Rattus norvegicus L-alanine + 4-methylsulfinyl-2-oxobutanoate
-
 ?
2.6.1.15 pyruvate + L-methionine sulfone poor substrate Rattus norvegicus L-alanine + 4-methylsulfonyl-2-oxobutanoate
-
 ?
2.6.1.15 pyruvate + L-phenylalanine
-
 Rattus norvegicus L-alanine + phenylpyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.15 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.15 60
-
 in crude extract inactivation within 10 min, in the presence of pyruvate, glyoxylate or phenylpyruvate 1 h stability, 25% loss of activity within 3 h Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.15 8.2 8.5
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.15 7.6 8.8 about half-maximum activity at pH 7.6 and about 90% of maximum activity at pH 8.8 Rattus norvegicus