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Literature summary extracted from

  • Samejima, K.; Yamanoha, B.
    Purification of spermidine synthase from rat ventral prostate by affinity chromatography on immobilized S-adenosyl(5)-3-thiopropylamine (1982), Arch. Biochem. Biophys., 216, 213-222.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.16 DTT stabilizes Rattus norvegicus
2.5.1.16 freeze-thawing inactivates Escherichia coli
2.5.1.16 freeze-thawing inactivates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 1,5-Diaminopentane weak, competitive Rattus norvegicus
2.5.1.16 5'-deoxy-5'-methylthioadenosine above 0.05 mM Rattus norvegicus
2.5.1.16 5'-deoxy-5'-methylthiotubercidine
-
 Rattus norvegicus
2.5.1.16 additional information no inhibition by 1,4-diaminobutane Escherichia coli
2.5.1.16 additional information no inhibition by putrescine up to 10 mM Rattus norvegicus
2.5.1.16 N-ethylmaleimide
-
 Escherichia coli
2.5.1.16 N-ethylmaleimide
-
 Rattus norvegicus
2.5.1.16 PCMB
-
 Rattus norvegicus
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine competitive substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.1
-
 1,4-diaminobutane pH 7.2, 37ºC Rattus norvegicus
2.5.1.16 1.1
-
 S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.2, 37ºC Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.16 K+ 10 mM, activity increases by 20% Rattus norvegicus
2.5.1.16 Mg2+ 10 mM, activity increases 20% Rattus norvegicus
2.5.1.16 Na+ 10 mM, activity increases by 20% Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 37000
-
 2 * 37000, SDS-PAGE Rattus norvegicus
2.5.1.16 73000
-
-
 Escherichia coli
2.5.1.16 73000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Escherichia coli
-
 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Rattus norvegicus
-
 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Rattus norvegicus Wistar
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Escherichia coli
-
-
-
2.5.1.16 Rattus norvegicus
-
 Wistar strain
-
2.5.1.16 Rattus norvegicus Wistar
-
 Wistar strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 affinity chromatography on S-adenosyl(5')-3-thiopropylamine-Sepharose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 prostate gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 1.3
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 0ºC, 30% loss of activity after 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane linear at least up to 6 nmol of spermidine formed, linearity not maintained in the absence of DTT Rattus norvegicus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane linear at least up to 6 nmol of spermidine formed, linearity not maintained in the absence of DTT Rattus norvegicus Wistar 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Rattus norvegicus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Rattus norvegicus Wistar 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
 Escherichia coli 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane aminopropylcadaverine ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane 20 times less active than 1,4-diaminobutane at the same concentration Rattus norvegicus 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane 20 times less active than 1,4-diaminobutane at the same concentration Rattus norvegicus Wistar 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer
-
 Escherichia coli
2.5.1.16 dimer 2 * 37000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Escherichia coli
2.5.1.16 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 additional information
-
 pI 5.3 Rattus norvegicus
2.5.1.16 10
-
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.16 10
-
 95% loss of activity after 30 min Rattus norvegicus
2.5.1.16 10.4
-
 above, rapid loss of activity Rattus norvegicus