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Literature summary extracted from

  • Bowman, W.H.; White Tabor, C.; Tabor, H.
    Spermidine biosynthesis. Purification and properties of propylamine transferase from Escherichia coli (1973), J. Biol. Chem., 248, 2480-2486.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.16 freeze-thawing inactivates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 5'-ethylthioadenosine
-
 Escherichia coli
2.5.1.16 additional information inhibition by end products of reaction; no inhibition by phenylhydrazine, semicarbazide, sodium borohydride, NaCN, KCl, NH4Cl, MgCl2, CaCl2, NaNO3, Na2SO4, Na2HPO4 Escherichia coli
2.5.1.16 N-ethylmaleimide
-
 Escherichia coli
2.5.1.16 p-hydroxymercuribenzoate 2-mercaptoethanol restores activity Escherichia coli
2.5.1.16 spermidine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.0022
-
 S-adenosyl-3-methylthio-1-propylamine pH 8.0, 37ºC, fluorometric assay Escherichia coli
2.5.1.16 0.012
-
 1,4-diaminobutane pH 8.8, 37ºC, spectrophotometric assay Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 70600
-
 W strain, sedimentation equilibrium centrifugation Escherichia coli
2.5.1.16 73000
-
 W strain Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Escherichia coli
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Escherichia coli
-
 W strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 2000fold purified Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 1.83
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 -20°C, partially purified enzyme, several weeks with less than 20% loss of activity Escherichia coli
2.5.1.16 0-4°C, purified enzyme, 10-20% loss of activity within 6 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ir
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine at a reduced rate Escherichia coli 5'-methylthioadenosine + spermine the reaction rate is slower than with 1,4-diaminobutane ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
 Escherichia coli 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer 2 * 30000-35000, SDS-PAGE Escherichia coli
2.5.1.16 dimer 2 * 37000-38000, high speed sedimentation equilibrium centrifugation in 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.16 50
-
 at least 30 min stable in 0.01 M Tris-HCl buffer, pH 8, 1 mM EDTA Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 10.4
-
 65% rate of reaction at pH 8.6 and 93% rate of reaction at pH 10.6 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.16 8.6 10.6 about 65% of maximal activity at pH 8.6 and about 90% of maximal activity at pH 10.6 Escherichia coli