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Literature summary extracted from

  • Triglia, T.; Menting, J.G.T.; Wilson, C.; Cowman, A.F.
    Mutations in dihydropteroate synthase are responsible for sulfone and sulfonamide resistance in Plasmodium falciparum (1997), Proc. Natl. Acad. Sci. USA, 94, 13944-13949.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 expression in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 dapsone
-
 Plasmodium falciparum
2.5.1.15 sulfadoxine
-
 Plasmodium falciparum
2.5.1.15 sulfamethoxazole
-
 Plasmodium falciparum
2.5.1.15 sulfathiazole competitive to 4-aminobenzoate Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 additional information
-
 additional information values for recombinant enzymes Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 222000
-
 gel filtration Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Plasmodium falciparum
-
 diphosphate + 7,8-dihydropteroate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 enzyme from recombinant clones in Sephacryl S-300 HR, enzyme isolated form Escherichia coli, several chromatographic columns Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Plasmodium falciparum diphosphate + 7,8-dihydropteroate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.15 37
-
 assay at Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 additional information
-
 additional information KI values of sulfadoxine for recombinant enzymes Plasmodium falciparum