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Literature summary extracted from

  • Richey, D.P.; Brown, G.M.
    Hydroxymethyldihydropteridine pyrophosphokinase and dihydropteroate synthetase from Escherichia coli (1971), Methods Enzymol., 18B, 765-771.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 7,8-Dihydropteroic acid
-
 Escherichia coli
2.5.1.15 sulfathiazole competitive to 4-aminobenzoate Escherichia coli
2.5.1.15 sulfonamide competitive to 4-aminobenzoate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.0025
-
 4-Aminobenzoic acid
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.15 Mg2+ required to synthesize product Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 52000
-
 polyacrylamide gel electrophoresis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Escherichia coli
-
 diphosphate + 7,8-dihydropteroate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 precipitation with ammonium sulfate followed by column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.15 30
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.15 -20°C, sucrose, indefinitely stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Escherichia coli diphosphate + 7,8-dihydropteroate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.15 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.15 100
-
 60 min, 75% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.15 8.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.15 7 10.5 70% of maximal activity at pH 7.0 and 10.5 Escherichia coli