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Literature summary extracted from

  • Richey, D.P.; Brown, G.M.
    The biosynthesis of folic acid. IX. Purification and properties of the enzymes required for the formation of dihydropteroic acid (1969), J. Biol. Chem., 244, 1582-1592.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 7,8-Dihydropteroic acid
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.0025
-
 4-Aminobenzoic acid
-
 Escherichia coli
2.7.6.3 0.015
-
 ATP pH 8.6, 37°C Escherichia coli
2.7.6.3 0.015
-
 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pH 8.6, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.15 Mg2+ required to synthesize product Escherichia coli
2.7.6.3 Mg2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 50000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Escherichia coli
-
-
-
2.7.6.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 gel filtration on Sephadex G-100 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.15 30
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Escherichia coli diphosphate + 7,8-dihydropteroate
-
 ?
2.7.6.3 ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
-
 Escherichia coli AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.15 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.15 100
-
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.15 8.5
-
-
 Escherichia coli