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Literature summary extracted from

  • Scocca, J.J.
    Pyrimidine nucleoside phosphorylase from Haemophilus influenzae (1978), Methods Enzymol., 51, 432-437.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.2 repeated cycles of freezing and thawing inactivate the enzyme if phosphate is absent Haemophilus influenzae
2.4.2.2 the enzyme is quite labile during the course of purification, dithiothreitol and glycerol are required for stability Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 0.03
-
 5-Bromouridine
-
 Haemophilus influenzae
2.4.2.2 0.07
-
 5-methyluridine
-
 Haemophilus influenzae
2.4.2.2 0.1
-
 5-bromodeoxyuridine
-
 Haemophilus influenzae
2.4.2.2 0.11
-
 thymidine
-
 Haemophilus influenzae
2.4.2.2 0.13
-
 deoxyuridine
-
 Haemophilus influenzae
2.4.2.2 0.24
-
 uridine
-
 Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.2
-
 Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.2 25.5
-
-
 Haemophilus influenzae

Storage Stability

EC Number Storage Stability Organism
2.4.2.2 -20°C, in presence of phosphate, glycerol and dithiothreitol, stable for 6 months Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 5-bromodeoxyuridine + phosphate 74% of the activity with uridine Haemophilus influenzae 5-bromouracil + deoxyribose 1-phosphate
-
 ?
2.4.2.2 5-bromouridine + phosphate 40% of the activity with uridine Haemophilus influenzae 5-bromouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-methyluridine + phosphate 27% of the activity with uridine Haemophilus influenzae 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate 12% of the activity with uridine Haemophilus influenzae uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate 21% of the activity with uridine Haemophilus influenzae thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 uracil arabinoside + phosphate
-
 Haemophilus influenzae uracil + alpha-D-arabinose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Haemophilus influenzae uracil + alpha-D-ribose 1-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.2 6.9
-
-
 Haemophilus influenzae
2.4.2.2 7.4
-
 ribonucleosides and uracil arabinoside Haemophilus influenzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.2 6.9 7.4 pH 6.9: optimum, pH 7.4: 75% of maximal activity Haemophilus influenzae