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Literature summary extracted from

  • Poulton, J.E.; Shin, S.I.
    Prunasin biosynthesis by cell-free extracts from black cherry (Prunus serotina Ehrh.) fruits and leaves (1983), Z. Naturforsch. C, 38, 369-374.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.178 0.32
-
 UDP-glucose assay at pH 7.3 in the presence of 20 mM of (R,S)-mandelonitrile. The enzyme exclusively utilizes the (R)-enantiomer Prunus serotina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.178 UDP-glucose + (R,S)-mandelonitrile Prunus serotina
-
 UDP + (R)-prunasin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.178 Prunus serotina
-
 wild black cherry
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.178 method that includes chromatography on Sephadex G-25 Prunus serotina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.178 0.001
-
 mandelic acid Prunus serotina
2.4.1.178 0.002
-
 benzyl alcohol Prunus serotina
2.4.1.178 0.0025
-
 mandelamide Prunus serotina
2.4.1.178 0.012
-
 S)-mandelonitrile Prunus serotina
2.4.1.178 0.018
-
 benzoic acid Prunus serotina

Storage Stability

EC Number Storage Stability Organism
2.4.1.178 -20°C, 10% glycerol, 72% of 2 days crude extract stable for several weeks Prunus serotina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.178 UDP-glucose + (R,S)-mandelonitrile
-
 Prunus serotina UDP + (R)-prunasin
-
 ?
2.4.1.178 UDP-glucose + benzoic acid
-
 Prunus serotina UDP + benzoyl-beta-D-glucoside
-
 ?
2.4.1.178 UDP-glucose + benzyl alcohol
-
 Prunus serotina UDP + benzyl-beta-D-glucoside
-
 ?
2.4.1.178 UDP-glucose + mandelamide
-
 Prunus serotina ?
-
 ?
2.4.1.178 UDP-glucose + mandelic acid
-
 Prunus serotina ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.178 7 8 highest rates are realized in Tris-HCl buffer at pH 7.5 Prunus serotina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.178 5.8 9.3 about 50% of activity maximum Prunus serotina