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Literature summary extracted from

  • Jorasch, P.; Warnecke, D.C.; Lindner, B.; Zahringer, U.; Heinz, E.
    Novel processive and nonprocessive glycosyltransferases from Staphylococcus aureus and Arabidopsis thaliana synthesize glycoglycerolipids, glycophospholipids, glycosphingolipids and glycosylsterols (2000), Eur. J. Biochem., 267, 3770-3783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.315 expression in Escherichia coli Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.315 44000
-
 x * 44000, SDS-PAGE, x * 44700, calculated Staphylococcus aureus
2.4.1.315 44700
-
 x * 44000, SDS-PAGE, x * 44700, calculated Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.315 Staphylococcus aureus Q5HH69
-
-
2.4.1.315 Staphylococcus aureus COL Q5HH69
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.315 GDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol GDP-glucose shows poor activity Staphylococcus aureus 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
2.4.1.315 GDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol GDP-glucose shows poor activity Staphylococcus aureus COL 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
2.4.1.315 additional information 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl]-sn-glycerol, i.e. diglucosyl diacylglycerol, is not further converted to triglucosyl diacylglycerol. Not accepted as substrates are UDP-galactose, UDP-mannose, GDP-mannose, UDP-xylose, and UDP-Nacetylglucosamine. Cholesterol, cholesterolglucoside and the fluorescent substrate 6-[[(N-7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl]sphingosine, i.e.NBD-ceramide, can alos serve as acceptors that are converted into more polar products Staphylococcus aureus ?
-
 ?
2.4.1.315 additional information 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl]-sn-glycerol, i.e. diglucosyl diacylglycerol, is not further converted to triglucosyl diacylglycerol. Not accepted as substrates are UDP-galactose, UDP-mannose, GDP-mannose, UDP-xylose, and UDP-Nacetylglucosamine. Cholesterol, cholesterolglucoside and the fluorescent substrate 6-[[(N-7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl]sphingosine, i.e.NBD-ceramide, can alos serve as acceptors that are converted into more polar products Staphylococcus aureus COL ?
-
 ?
2.4.1.315 UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 Staphylococcus aureus 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
2.4.1.315 UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 Staphylococcus aureus COL 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
2.4.1.315 UDP-alpha-D-glucose + glycero-3-phospho-(1'-sn-glycerol)
-
 Staphylococcus aureus alpha-D-glucopyranosyl-glycero-3-phospho-(1'-sn-glycerol) + UDP
-
 ?
2.4.1.315 UDP-alpha-D-glucose + glycero-3-phospho-(1'-sn-glycerol)
-
 Staphylococcus aureus COL alpha-D-glucopyranosyl-glycero-3-phospho-(1'-sn-glycerol) + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.315 ? x * 44000, SDS-PAGE, x * 44700, calculated Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.315 beta-diglucosyldiacylglycerol synthase
-
 Staphylococcus aureus
2.4.1.315 processive diacylglycerol beta-glucosyltransferase
-
 Staphylococcus aureus
2.4.1.315 UgtP
-
 Staphylococcus aureus