Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marchase, R.B.; Bounelis, P.; Brumley, L.M.; Dey, N.; Browne, B.; Auger, D.; Fritz, T.A.; Kulesza, P.; Bedwell, D.M.
    Phosphoglucomutase in Saccharomyces cerevisiae is a cytoplasmic glycoprotein and the acceptor for a Glc-phosphotransferase (1993), J. Biol. Chem., 268, 8341-8349.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.8.19 active fractions are frozen in a dry ice/methanol bath immediately after preparation and stored at -80°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.19 membrane
-
 Rattus norvegicus 16020
-
2.7.8.19 membrane membrane-associated enzyme, faces the cytosol Saccharomyces cerevisiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.19 Ca2+ added in the activity assay at a final concentration of 5 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.19 UDP-glucose + phosphoglucomutase Saccharomyces cerevisiae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.19 Rattus norvegicus
-
-
-
2.7.8.19 Saccharomyces cerevisiae
-
 strains NY13 and YDB135
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.19 additional information GlcPTase from Saccharomyces cerevisiae recognizes and reacts with GlcPTase acceptor from Rattus norvegicus. The reciprocal experiment also results in glucose phosphate transfer demonstrating that this glycosylation reaction has been conserved during the evolution. Phosphoglucomutase from rabbit muscle can also act as an acceptor Rattus norvegicus ?
-
 ?
2.7.8.19 additional information GlcPTase from Saccharomyces cerevisiae recognizes and reacts with GlcPTase acceptor from Rattus norvegicus. The reciprocal experiment also results in glucose phosphate transfer demonstrating that this glycosylation reaction has been conserved during the evolution. Phosphoglucomutase from rabbit muscle can also act as an acceptor Saccharomyces cerevisiae ?
-
 ?
2.7.8.19 UDP-glucose + glycoprotein D-mannose
-
 Rattus norvegicus UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
-
 ?
2.7.8.19 UDP-glucose + glycoprotein D-mannose
-
 Saccharomyces cerevisiae UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
-
 ?
2.7.8.19 UDP-glucose + phosphoglucomutase
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.8.19 UDP-glucose + phosphoglucomutase specific for UDP-glucose, the site affected in the GlcPTase reaction is not the enzymatic active site of phosphoglucomutase, underglucosylated forms of phosphoglucomutase obtained in response to heat shock and growth in media containing carbon sources other than glucose increase transfer in vitro GlcPTase assays and appear to reflect regulation of the GlcPTase activity Saccharomyces cerevisiae ?
-
 ?