Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schut, G.J.; Menon, A.L.; Adams, M.W.W.
    2-Keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis (2001), Methods Enzymol., 331, 144-158.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.7 thiamine diphosphate requires for maximal activity of enzyme Pyrococcus furiosus
1.2.7.7 thiamine diphosphate requires for maximal activity of enzyme Thermococcus litoralis
1.2.7.8 thiamine diphosphate
-
Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.7
-
Pyrococcus furiosus
1.2.7.8
-
Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.7 additional information
-
additional information
-
Pyrococcus furiosus
1.2.7.7 additional information
-
additional information
-
Thermococcus litoralis
1.2.7.8 0.017
-
CoA
-
Pyrococcus furiosus
1.2.7.8 0.048
-
Ferredoxin
-
Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.7 Fe each of the tetramer contains three [4Fe-4S] cluster Pyrococcus furiosus
1.2.7.7 Fe each of the tetramer contains three [4Fe-4S] cluster Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.7 13000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 23000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 34000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.7 47000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.8 23000
-
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 24000
-
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 66000
-
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 71000
-
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.7 additional information Pyrococcus furiosus enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
?
1.2.7.7 additional information Thermococcus litoralis enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
?
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Methanothermobacter thermautotrophicus
-
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Pyrococcus furiosus
-
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.7 Pyrococcus furiosus
-
-
-
1.2.7.7 Thermococcus litoralis
-
-
-
1.2.7.8 Methanothermobacter thermautotrophicus
-
-
-
1.2.7.8 Pyrococcus furiosus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.7 enzyme is irreversibly inactivated by oxygen Pyrococcus furiosus
1.2.7.8 irreversibly inactivated by oxygen Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.7 under anaerobic conditions Pyrococcus furiosus
1.2.7.8
-
Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ pathway Pyrococcus furiosus
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ pathway Thermococcus litoralis
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus furiosus
1.2.7.7 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus propanoyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 2-ketobutyrate + CoA + oxidized ferredoxin
-
Thermococcus litoralis propanoyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Thermococcus litoralis S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Thermococcus litoralis S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
1.2.7.7 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 indolpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus indolacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 indolpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis indolacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus furiosus ?
-
?
1.2.7.7 additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus litoralis ?
-
?
1.2.7.7 additional information enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde Pyrococcus furiosus ?
-
?
1.2.7.7 additional information enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde Thermococcus litoralis ?
-
?
1.2.7.7 additional information no substrate is 2-ketoglutarate Pyrococcus furiosus ?
-
?
1.2.7.7 additional information no substrate is 2-ketoglutarate Thermococcus litoralis ?
-
?
1.2.7.7 phenylglyoxylate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus benzoyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 phenylglyoxylate + CoA + oxidized ferredoxin
-
Thermococcus litoralis benzoyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.7 pyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
Methanothermobacter thermautotrophicus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+
-
r
1.2.7.8 2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA
-
r
1.2.7.8 2-ketoisocaproate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus CO2 + reduced ferredoxin + isovaleryl-CoA
-
r
1.2.7.8 indolepyruvate + CoA + oxidized methylviologen
-
Pyrococcus furiosus indoleacetyl-CoA + CO2 + reduced methylviologen
-
?
1.2.7.8 additional information pyruvate, 2-ketoisovalerate, 2-ketoglutarate, 2 ketobutyrate, and phenylglyoxylate are no substrates Pyrococcus furiosus ?
-
?
1.2.7.8 p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
Methanothermobacter thermautotrophicus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r

Subunits

EC Number Subunits Comment Organism
1.2.7.7 octamer alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
1.2.7.8 tetramer
-
Methanothermobacter thermautotrophicus
1.2.7.8 tetramer 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 tetramer 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus