EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.7.4 | gene lytB, expression as His-tagged enzyme in Escherichia coli strain XL1-Blue | Aquifex aeolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.7.4 | 0.59 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | recombinant enzyme, pH 7.5, 60°C | Aquifex aeolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Aquifex aeolicus | - |
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O | Aquifex aeolicus | part of isoprenoid biosynthesis | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O | Aquifex aeolicus | 2-C-methyl-D-erithritol-4-phosphate pathway | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.7.4 | Aquifex aeolicus | O67625 | gene lytB | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.17.7.4 | highly sensitive to O2, 21% remaining activity after incubation in air for 10 min | Aquifex aeolicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.7.4 | recombinant His-tagged protein from Escherichia coli strain Xl1-Blue, to 95% homogeneity | Aquifex aeolicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.17.7.4 | isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | reaction mechanism | Aquifex aeolicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 6.6 | - |
recombinant enzyme | Aquifex aeolicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.17.7.4 | -20°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 95% remaining activity after 2 weeks | Aquifex aeolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Aquifex aeolicus | isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | producing a 5:1 mixture of isopentenyl diphosphate and dimethyallyl diphosphate | r | |
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Aquifex aeolicus | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Aquifex aeolicus | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | producing a 5:1 mixture of isopentenyl diphosphate and dimethyallyl diphosphate | r | |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O | part of isoprenoid biosynthesis | Aquifex aeolicus | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O | 2-C-methyl-D-erithritol-4-phosphate pathway | Aquifex aeolicus | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 60 | - |
- |
Aquifex aeolicus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 48 | 72 | half-maximal activity at 48°C and 72°C | Aquifex aeolicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 65 | - |
stable | Aquifex aeolicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.7.4 | 3.7 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | recombinant enzyme, pH 7.5, 60°C | Aquifex aeolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 7 | 7.5 | - |
Aquifex aeolicus |