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Literature summary extracted from

  • Rohdich, F.; Hecht, S.; Gärtner, K.; Adam, P.; Krieger, C.; Amslinger, S.; Arigoni, D.; Bacher, A.; Eisenreich, W.
    Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein (2002), Proc. Natl. Acad. Sci. USA, 99, 1158-1163.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.4 gene ispH, expression as His-tagged protein in Escherichia coli strain XL1-Blue, construction of a synthetic operon containing ispH, ispG and gepE Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.7.4 37000
-
x * 37000, recombinant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Escherichia coli
-
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
r
1.17.7.4 isopentenyl diphosphate + NAD(P)+ + H2O Escherichia coli biosynthesis of terpenes (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H
-
r
1.17.7.4 isopentenyl diphosphate + NAD(P)+ + H2O Escherichia coli investigation of biosynthetic coupling and pattern by expression of involved genes from synthetic operon (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.4 Escherichia coli P62623 gene ispH, formerly lytB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.7.4 recombinant His-tagged protein from Escherichia coli strain XL1-Blue, to homogeneity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Escherichia coli isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
r
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Escherichia coli dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
r
1.17.7.4 isopentenyl diphosphate + NAD(P)+ + H2O biosynthesis of terpenes Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H
-
r
1.17.7.4 isopentenyl diphosphate + NAD(P)+ + H2O investigation of biosynthetic coupling and pattern by expression of involved genes from synthetic operon Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H
-
r

Subunits

EC Number Subunits Comment Organism
1.17.7.4 ? x * 36161-36173, recombinant enzyme, DNA sequence determination and electrospray mass spectroscopy Escherichia coli
1.17.7.4 ? x * 37000, recombinant enzyme, SDS-PAGE Escherichia coli