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Literature summary extracted from

  • Gollub, M.; Shaw, L.
    Isolation and characterization of cytidine-5'-monophosphate-N-acetylneuraminate hydroxylase from the starfish Asterias rubens (2003), Comp. Biochem. Physiol. B, 134, 89-101.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.18.2 after each cycle of freezing and thawing, 25% loss of activity Asterias rubens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.2 1,10-phenanthroline
-
 Asterias rubens
1.14.18.2 2,2'-dipyridyl
-
 Asterias rubens
1.14.18.2 ferrozine
-
 Asterias rubens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.2 NaCl maximal 3.5fold stimulation at 100 mM Asterias rubens

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Asterias rubens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.2
-
 Asterias rubens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 gonad
-
 Asterias rubens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.2 0.00093
-
-
 Asterias rubens

Storage Stability

EC Number Storage Stability Organism
1.14.18.2 -80 C, very stable Asterias rubens
1.14.18.2 4°C, 48 h, 10% loss of activity Asterias rubens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 evidence for the formation of a ternary complex of hydroxylase, CMP-Neu5Ac and cytochrome b5 Asterias rubens CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
 ?
1.14.18.2 CMP-N-acetylneuraminate + NADH + O2
-
 Asterias rubens CMP-N-glycoloylneuraminate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.2 More a main protein of 76000 da and a minor protein of 64000 Da correlates with CMP-neu5Ac hydroxylase activity Asterias rubens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.2 4
-
 48 h, 10% loss of activity Asterias rubens
1.14.18.2 25
-
 2 h, 50% loss of activity Asterias rubens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.2 ferrocytochrome b5 evidence for the formation of a ternary complex of hydroxylase, CMP-Neu5Ac and cytochrome b5 Asterias rubens
1.14.18.2 NADH
-
 Asterias rubens