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Literature summary extracted from

  • Schneckenburger, P.; Shaw, L.; Schauer, R.
    Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver (1994), Glycoconjugate J., 11, 194-203.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.2 0.005
-
 CMP-N-acetylneuraminate pH 7.4, 37°C, amphiphilic system Mus musculus
1.14.18.2 0.013
-
 CMP-N-acetylneuraminate pH 7.4, 37°C, soluble system Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.2 microsome
-
 Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.2 58000
-
 gel filtration Mus musculus
1.14.18.2 64000
-
 1 * 64000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + NADH + O2 Mus musculus a regulation of CMP-N-acetylneuraminate hydroxylation and thus the ratio of glycoconjugate-bound N-acetylneuraminate and N-acetylglycoloylneuraminate might occur by varying the amount of hydroxylase protein within the cell, possibly by controlling the expression of the hydroxylase gene CMP-N-glycoloylneuraminate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.2
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.2 0.126
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + ferrocytochrome b5 + O2
-
 Mus musculus CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
 ?
1.14.18.2 CMP-N-acetylneuraminate + NADH + O2
-
 Mus musculus CMP-N-glycoloylneuraminate + NAD+ + H2O
-
 ?
1.14.18.2 CMP-N-acetylneuraminate + NADH + O2 a regulation of CMP-N-acetylneuraminate hydroxylation and thus the ratio of glycoconjugate-bound N-acetylneuraminate and N-acetylglycoloylneuraminate might occur by varying the amount of hydroxylase protein within the cell, possibly by controlling the expression of the hydroxylase gene Mus musculus CMP-N-glycoloylneuraminate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.2 monomer 1 * 64000, SDS-PAGE Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.2 NADH
-
 Mus musculus