EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.17.4 | wild-type enzyme and mutants, expression in Escherichia coli BL21(DE3) | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.17.4 | D179E | site-directed mutagenesis, very low activity, stimulation by bicarbonate | Solanum lycopersicum |
1.14.17.4 | D179N | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H177D | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H177D/D179E | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H177E | site-directed mutagenesis, very low activity, stimulation by bicarbonate | Solanum lycopersicum |
1.14.17.4 | H177Q | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H211Q | site-directed mutagenesis, slightly reduced activity | Solanum lycopersicum |
1.14.17.4 | H234D | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H234E | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H234Q | site-directed mutagenesis, no activity | Solanum lycopersicum |
1.14.17.4 | H39Q | site-directed mutagenesis, slightly reduced activity | Solanum lycopersicum |
1.14.17.4 | H56Q | site-directed mutagenesis, activity similar to the wild-type | Solanum lycopersicum |
1.14.17.4 | H94Q | site-directed mutagenesis, slightly reduced activity | Solanum lycopersicum |
EC Number | General Stability | Organism |
---|---|---|
1.14.17.4 | enzyme has a short half-life under catalytic conditions undergoing metal-catalyzed oxidative fragmentation | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.17.4 | 0.00029 | - |
Fe2+ | recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.00043 | - |
Fe2+ | recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.00047 | - |
Fe2+ | recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.0046 | - |
Fe2+ | recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.042 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.083 | - |
1-aminocyclopropane-1-carboxylate | recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.121 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.132 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.4 | Fe2+ | required | Solanum lycopersicum | |
1.14.17.4 | Fe2+ | iron binding ligands are His177, Asp179, His234 | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Solanum lycopersicum | biosynthesis of plant signalling molecule ethylene | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.17.4 | Solanum lycopersicum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.17.4 | recombinant wild-type enzyme and mutants from Escherichia coli BL21(DE3) to near homogeneity | Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | biosynthesis of plant signalling molecule ethylene | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1.14.17.4 | 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.17.4 | 28 | - |
assay at | Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.17.4 | 0.00867 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 0.0095 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 5.17 | - |
1-aminocyclopropane-1-carboxylate | recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.14.17.4 | 5.9 | - |
1-aminocyclopropane-1-carboxylate | recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.17.4 | 7.2 | - |
assay at | Solanum lycopersicum |