EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.17.4 | expression of wild-type and mutants in Escherichia coli | Actinidia deliciosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.17.4 | D179E | site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
1.14.17.4 | G137P | site-directed mutagenesis, 98% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
1.14.17.4 | H177Q | site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
1.14.17.4 | K158A | site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
1.14.17.4 | K158C | site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
1.14.17.4 | K172A | site-directed mutagenesis, 85% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
1.14.17.4 | K172C | site-directed mutagenesis, 64% activity compared to the recombinant wild-type enzyme | Actinidia deliciosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Actinidia deliciosa | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.17.4 | Actinidia deliciosa | P31237 | kiwi | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.17.4 | recombinant wild-type enzyme and mutants from Escherichia coli | Actinidia deliciosa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.17.4 | fruit | - |
Actinidia deliciosa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.17.4 | 0.073 | - |
purified recombinant mutant K172C | Actinidia deliciosa |
1.14.17.4 | 0.1 | - |
purified recombinant mutant K172A | Actinidia deliciosa |
1.14.17.4 | 0.11 | - |
purified recombinant mutant G137P | Actinidia deliciosa |
1.14.17.4 | 0.16 | - |
purified recombinant wild-type enzyme | Actinidia deliciosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Actinidia deliciosa | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1.14.17.4 | 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Actinidia deliciosa | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |