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Literature summary extracted from

  • Zhang, Z.; Ren, J.; Stammers, D.K.; Baldwin, J.E.; Harlos, K.; Schofield, C.J.
    Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase (2000), Nat. Struct. Biol., 7, 127-133.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.11.21 recombinant isozyme CAS1, hanging drop method, 2 M ammonium sulfate, 0.1 M Tris, 21°C, apoform crystals or in complex with Fe2+, N-alpha-acetyl-L-arginine, and the substrates 2-oxoglutarate and proclavaminate, X-ray diffraction structure determination for native and heavy atom derivatives and analysis Streptomyces clavuligerus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.21 Fe2+
-
 Streptomyces clavuligerus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.21 Streptomyces clavuligerus Q05581 recombinant CAS1
-
1.14.11.21 Streptomyces clavuligerus Q05581 crystal structure of isozyme CAS1, complexed or uncomplexed, recombinant from Escherichia coli, Protein Data Bank: 1DS0, 1DS1, 1DRY, 1DRT, 1GVG
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.11.21 dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a
1.14.11.21 dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O substrate-binding mechanism Streptomyces clavuligerus
a
1.14.11.21 proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a
1.14.11.21 proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O substrate-binding mechanism Streptomyces clavuligerus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.21 deoxyamidinoproclavaminate + 2-oxoglutarate + O2 this reaction is due to a proclavaminate amidinohydrolase, i.e. cla Streptomyces clavuligerus amidinoproclavaminate + succinate + CO2 + H2O
-
 ?
1.14.11.21 deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
1.14.11.21 dihydroclavaminate + 2-oxoglutarate + O2 cyclization Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
 ?
1.14.11.21 additional information substrate-binding Streptomyces clavuligerus ?
-
 ?
1.14.11.21 additional information dependent on O2 and 2-oxoglutarate Streptomyces clavuligerus ?
-
 ?
1.14.11.21 N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
1.14.11.21 N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 isozyme CAS1 Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
1.14.11.21 proclavaminate + 2-oxoglutarate + O2 saturation Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.21 CAS
-
 Streptomyces clavuligerus