EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.21 | isozymes CS1 and CS2, overexpression in Escherichia coli JM101 | Streptomyces clavuligerus |
EC Number | General Stability | Organism |
---|---|---|
1.14.11.21 | Co2+ completely inhibits the enzyme, competitive to Fe2+, but also stabilizes the enzyme against self-inactivation in absence of proclavaminate | Streptomyces clavuligerus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.21 | Co2+ | complete inhibition competitive to Fe2+, but stabilizes the enzyme against self-inactivation in absence of proclavaminate | Streptomyces clavuligerus | |
1.14.11.21 | diethyl dicarbonate | isozyme CS2, rapid inactivation at pH 6.0, 25°C, t1/2: 1.1 min | Streptomyces clavuligerus | |
1.14.11.21 | N-ethylmaleimide | inactivation | Streptomyces clavuligerus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.21 | 0.13 | - |
proclavaminate | isozyme CS2, pH 7.0, 22°C | Streptomyces clavuligerus | |
1.14.11.21 | 0.22 | - |
proclavaminate | isozyme CS1, pH 7.0, 22°C | Streptomyces clavuligerus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.21 | Fe2+ | required | Streptomyces clavuligerus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.21 | 35360 | - |
recombinant enzyme | Streptomyces clavuligerus |
1.14.11.21 | 35360 | - |
CS1, mass spectrometry | Streptomyces clavuligerus |
1.14.11.21 | 35750 | - |
recombinant enzyme | Streptomyces clavuligerus |
1.14.11.21 | 35750 | - |
CS2, mass spectrometry | Streptomyces clavuligerus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
guanidinoproclavaminate + succinate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.21 | Streptomyces clavuligerus | - |
2 isozymes CS1 and CS2 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.14.11.21 | inactivation due to release of peroxide from reaction of 2-oxoglutarate and O2 in absence of proclavaminate, in presence of Fe2+, t1/2: 5 min, with ascorbate instead of 2-oxoglutarate t1/2: 50 min, catalase protects | Streptomyces clavuligerus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.21 | recombinant isozymes CS1 and CS2 to homogeneity, 10fold | Streptomyces clavuligerus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus | |
1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.11.21 | 0.8 | - |
purified recombinant isozyme CS2 | Streptomyces clavuligerus |
1.14.11.21 | 0.86 | - |
purified recombinant isozyme CS1 | Streptomyces clavuligerus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
1.14.11.21 | additional information | 1 or more histidine as well as cysteine residue located at or near the active site or in the metal binding site, critical for catalysis | Streptomyces clavuligerus | ? | - |
? | |
1.14.11.21 | additional information | dependent on O2 and 2-oxoglutarate | Streptomyces clavuligerus | ? | - |
? | |
1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.21 | CS | - |
Streptomyces clavuligerus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.21 | 7 | - |
assay at | Streptomyces clavuligerus |