EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.2 | Acinetobacter calcoaceticus | - |
- |
- |
1.1.99.35 | Acinetobacter calcoaceticus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | additional information | absolute specificity with respect to the C1 position, only sugars are oxidized which have the same configuration of the H/OH substituents at this site as the beta-anomer of glucose. Absolute specificity with respect to the overall conformation of the sugar molecule, sugars with a 4C1 chair conformation are substrates, those with a 1C4 one are not. The nature and configuration of the substituents at the 3-position are hardly relevant for activity, and an equatorial pyranose group at the 4-position exhibits only a specific hindering of the binding of the aldose moiety of a disaccharide | Acinetobacter calcoaceticus | ? | - |
? | |
1.1.99.35 | 2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | cellobiose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-allose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-fucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-fucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-galactose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-lyxose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-lyxono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-mannose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-ribose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-xylose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | L-arabinose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | lactose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | maltose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | melibiose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | additional information | kinetic mechanism of sGDH consists of (a) step(s) in which a fluorescing intermediate is formed, and a subsequent, irreversible step, determining the overall rate of the reductive half-reaction. The pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions. | Acinetobacter calcoaceticus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.35 | sGDH | - |
Acinetobacter calcoaceticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | additional information | - |
additional information | - |
Acinetobacter calcoaceticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 7 | - |
D-glucose oxidation | Acinetobacter calcoaceticus |
1.1.99.35 | 7 | - |
pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions | Acinetobacter calcoaceticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.35 | 0.24 | - |
D-Lyxose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 11 | - |
D-ribose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 11 | - |
melibiose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 45 | - |
D-xylose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 46 | - |
D-xylose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 50 | - |
D-mannose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 150 | - |
D-galactose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 240 | - |
D-galactose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 440 | - |
D-fucose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 790 | - |
lactose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 800 | - |
maltose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 960 | - |
lactose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 1080 | - |
cellobiose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 1450 | - |
L-arabinose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 1470 | - |
D-allose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 1700 | - |
D-glucose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 2100 | - |
D-glucose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus |