Literature summary extracted from

Searles, L.L.; Calvo, J.M.
Permeabilized cell and radiochemical assay for beta-isopropylmalate dehydrogenase (1988), Methods Enzymol., 166, 225-229.

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.85	Escherichia coli	-	-	-
1.1.1.85	Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.85	additional information	-	radiochemical assay	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.85	(2R,3S)-3-isopropylmalate + NAD+	-	Salmonella enterica subsp. enterica serovar Typhimurium	2-oxoisocaproate + NADH + H+ + CO2	-	?
1.1.1.85	(2R,3S)-3-isopropylmalate + NAD+	-	Escherichia coli	2-oxoisocaproate + NADH + H+ + CO2	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.85	NAD+	-	Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85	NAD+	-	Escherichia coli

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Release 2023.1 (January 2023)