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Literature summary extracted from

  • Parsons, S.J.; Burns, R.O.
    beta-Isopropylmalate dehydrogenase (Salmonella typhimurium) (1970), Methods Enzymol., 17A, 793-799.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.85 dilute solutions, 1 mg/ml or less, precipitate upon thawing Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.85 Ba2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 Ca2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 Co2+ above 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 iodoacetamide 50 mM, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 iodoacetate 50 mM, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 K+ above 1 M, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 Li+
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 Mn2+ above 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 N-ethylmaleimide
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 NEM 5 mM, 50% inhibition, not reversed by 2-mercaptoethanol Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 p-hydroxymercuribenzoate pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 PCMB
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.019
-
(2R,3S)-3-isopropylmalate pH 9.0 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 0.1
-
NAD+ pH 9.0 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.85 Co2+ divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 K+ monovalent cation required, maximal activity at 0.05 M Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 Mg2+ divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 Mn2+ divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 NH4+ monovalent cations required, NH4+ can partially replace K+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.85 35000
-
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 70000
-
equilibrium sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Salmonella enterica subsp. enterica serovar Typhimurium penultimate step in leucine biosynthesis 2-oxoisocaproate + NADH + H+ + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Salmonella enterica subsp. enterica serovar Typhimurium
-
strain leu A-124
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.85 54
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
1.1.1.85 -20°C, pH 5.5-7.5, fully active Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisocaproate + NADH + H+ + CO2
-
?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ penultimate step in leucine biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisocaproate + NADH + H+ + CO2
-
?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
r
1.1.1.85 (2R,3S)-3-isopropylmalate + thionicotinamide NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisohexanoate + thionicotinamideNADH + CO2
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.85 dimer 2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.85 9 9.5
-
Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.85 5.5 7.5 stable if stored concentrated Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium