EC Number | General Stability | Organism |
---|---|---|
1.1.1.85 | dilute solutions, 1 mg/ml or less, precipitate upon thawing | Salmonella enterica subsp. enterica serovar Typhimurium |
1.1.1.85 | slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | Ba2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | Ca2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | Co2+ | above 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | iodoacetamide | 50 mM, slight inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | iodoacetate | 50 mM, slight inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | K+ | above 1 M, slight inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | Li+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | Mn2+ | above 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | N-ethylmaleimide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | NEM | 5 mM, 50% inhibition, not reversed by 2-mercaptoethanol | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | p-hydroxymercuribenzoate | pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | PCMB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.85 | 0.019 | - |
(2R,3S)-3-isopropylmalate | pH 9.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | 0.1 | - |
NAD+ | pH 9.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | Co2+ | divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | K+ | monovalent cation required, maximal activity at 0.05 M | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | Mg2+ | divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | Mn2+ | divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.85 | NH4+ | monovalent cations required, NH4+ can partially replace K+ | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | 35000 | - |
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl | Salmonella enterica subsp. enterica serovar Typhimurium |
1.1.1.85 | 70000 | - |
equilibrium sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | Salmonella enterica subsp. enterica serovar Typhimurium | penultimate step in leucine biosynthesis | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.85 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
strain leu A-124 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.85 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | 54 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.85 | -20°C, pH 5.5-7.5, fully active | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | penultimate step in leucine biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
r | |
1.1.1.85 | (2R,3S)-3-isopropylmalate + thionicotinamide NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoisohexanoate + thionicotinamideNADH + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.85 | dimer | 2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | 9 | 9.5 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | 5.5 | 7.5 | stable if stored concentrated | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium |