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Literature summary extracted from

  • Smith, L.D.; Budgen, N.; Bungard, S.J.; Danson, M.J.; Hough, D.W.
    Purification and characterization of glucose dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum (1989), Biochem. J., 261, 973-977.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.47 4 M urea, room temperature, 6 h, 100% activity Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.359 acetone 40% (v/v), 40% activity Thermoplasma acidophilum
1.1.1.359 ethanol 40% (v/v), 30% activity Thermoplasma acidophilum
1.1.1.359 methanol 40% (v/v), 50% activity Thermoplasma acidophilum
1.1.1.359 Urea 40% (v/v), 85% activity Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.113
-
 NADP+ pH 7.0, 55°C Thermoplasma acidophilum
1.1.1.47 10
-
 beta-D-glucose pH 7.0, 55°C Thermoplasma acidophilum
1.1.1.359 additional information
-
 NAD+ Km is superior to 30 mM. The Km value for NAD+ can not be defined precisely as it is impossible to reach saturation of the enzyme Thermoplasma acidophilum
1.1.1.359 0.113
-
 NADP+ pH 7.0, 55°C Thermoplasma acidophilum
1.1.1.359 10
-
 beta-D-glucose pH 7.0, 55°C Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 38000
-
 4 * 38000, SDS-PAGE Thermoplasma acidophilum
1.1.1.47 155000
-
 gel filtration Thermoplasma acidophilum
1.1.1.359 38000
-
 4 * 38000 Thermoplasma acidophilum
1.1.1.359 155000
-
 gel filtration Thermoplasma acidophilum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.47 Acetone 50% v/v, room temperature, 6 h, stable Thermoplasma acidophilum
1.1.1.47 Ethanol 50% v/v, room temperature, 6 h, stable Thermoplasma acidophilum
1.1.1.47 Methanol 50% v/v, room temperature, 6 h, stable Thermoplasma acidophilum
1.1.1.359 Acetone 50% (v/v), no appreciable loss of activity Thermoplasma acidophilum
1.1.1.359 Ethanol 50% (v/v), no appreciable loss of activity Thermoplasma acidophilum
1.1.1.359 Methanol 50% (v/v), no appreciable loss of activity Thermoplasma acidophilum
1.1.1.359 urea 4 M, no appreciable loss of activity Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Thermoplasma acidophilum
-
-
-
1.1.1.359 Thermoplasma acidophilum P13203
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 methanol extraction, Mono Q, Matrex Gel Red A, Mono P, gel filtration Thermoplasma acidophilum
1.1.1.359
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 320
-
-
 Thermoplasma acidophilum
1.1.1.359 320
-
 pH 7.0, 55°C Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD+
-
 Thermoplasma acidophilum D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+
-
 Thermoplasma acidophilum D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.359 beta-D-glucose + NAD+ + H2O
-
 Thermoplasma acidophilum D-gluconate + NADH + 2 H+
-
 ir
1.1.1.359 beta-D-glucose + NADP+ + H2O
-
 Thermoplasma acidophilum D-gluconate + NADPH + 2 H+
-
 ir
1.1.1.359 D-galactose + NAD+ + H2O
-
 Thermoplasma acidophilum D-galactonate + NADH + 2 H+
-
 ?
1.1.1.359 D-galactose + NADP+ + H2O the activity is 70% of the activity with beta-D-glucose + NADP+ Thermoplasma acidophilum D-galactonate + NADPH + 2 H+
-
 ?
1.1.1.359 additional information no activity with mannose or glucose 6-phosphate Thermoplasma acidophilum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 tetramer 4 * 38000, SDS-PAGE Thermoplasma acidophilum
1.1.1.359 tetramer 4 * 38000 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.359 55
-
 assay at Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 55
-
 9 h, stable Thermoplasma acidophilum
1.1.1.47 75
-
 half-life: approximately 3 h Thermoplasma acidophilum
1.1.1.359 55
-
 catalytic activity is retained after 9 h Thermoplasma acidophilum
1.1.1.359 75
-
 half-life: 3 h Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.359 7
-
 assay at Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
 Thermoplasma acidophilum
1.1.1.47 NADP+
-
 Thermoplasma acidophilum
1.1.1.359 NAD+ the enzyme is catalytically active with both NADP+ and NAD+. NADP+ and NAD+ bind at the same site on the enzyme Thermoplasma acidophilum
1.1.1.359 NADP+ the enzyme is catalytically active with both NADP+ and NAD+. NADP+ and NAD+ bind at the same site on the enzyme Thermoplasma acidophilum