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Literature summary extracted from

  • Warneck, H.M.; Seitz, H.U.
    3beta-Hydroxysteroid oxidoreductase in suspension cultures of Digitalis lanata EHRH (1990), Z. Naturforsch. C, 45, 963-972.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.278 DTT 1 mM, 1.75fold increase in activity Digitalis lanata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.278 2-mercaptoethanol activates at 1 mM, inhibitory at 10 mM Digitalis lanata
1.1.1.278 ascorbate
-
 Digitalis lanata
1.1.1.278 Ca2+
-
 Digitalis lanata
1.1.1.278 Co2+
-
 Digitalis lanata
1.1.1.278 L-cysteine 1 mM, together with 2-mercaptoethanl, up to 50% inhibition Digitalis lanata
1.1.1.278 Mg2+
-
 Digitalis lanata
1.1.1.278 Mn2+
-
 Digitalis lanata
1.1.1.278 NADPH inhibitory at concentrations above 1 mM Digitalis lanata
1.1.1.278 Zn2+
-
 Digitalis lanata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.278 0.0185 0.02 5alpha-pregnan-3,20-dione pH 8.0, 25°C Digitalis lanata
1.1.1.278 0.05 0.12 NADPH pH 8.0, 25°C Digitalis lanata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.278 cytosol major part Digitalis lanata 5829
-
1.1.1.278 endoplasmic reticulum minor part Digitalis lanata 5783
-
1.1.1.278 microsome
-
 Digitalis lanata
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.278 5alpha-pregnan-3,20-dione + NADPH Digitalis lanata
-
 3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.278 Digitalis lanata
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.278 cell suspension culture
-
 Digitalis lanata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.278 5alpha-pregnan-3,20-dione + NAD(P)H
-
 Digitalis lanata 3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+ i.e. 5alpha-pregnan-3beta-ol-20-one r
1.1.1.278 5alpha-pregnan-3,20-dione + NADPH
-
 Digitalis lanata 3beta-hydroxy-5alpha-pregnane-20-one + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.278 3beta-hydroxysteroid oxidoreductase
-
 Digitalis lanata
1.1.1.278 More 3beta-hydroxysteroid oxidoreductase is exclusively associated with the 5alpha-progesterone reductase in microsomes Digitalis lanata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.278 25
-
-
 Digitalis lanata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.278 5 35 half-maximal activity at 5°C and 35°C Digitalis lanata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.278 8
-
-
 Digitalis lanata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.278 6 9
-
 Digitalis lanata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.278 NAD+
-
 Digitalis lanata
1.1.1.278 NADH
-
 Digitalis lanata
1.1.1.278 NADP+
-
 Digitalis lanata
1.1.1.278 NADPH required Digitalis lanata