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Literature summary extracted from

  • Taylor, J.C.; Takusagawa, F.; Markham, G.D.
    The active site loop of S-adenosylmethionine synthetase modulates catalytic efficiency (2002), Biochemistry, 41, 9358-9369.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.0013
-
tripolyphosphate wild type, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine Escherichia coli
2.5.1.6 0.0015
-
tripolyphosphate G7 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine Escherichia coli
2.5.1.6 0.0016
-
tripolyphosphate G8 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine Escherichia coli
2.5.1.6 0.003
-
tripolyphosphate wild type, tripolyphosphatase activity, in absence of S-adenosylmethionine Escherichia coli
2.5.1.6 0.0032
-
tripolyphosphate G6 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine Escherichia coli
2.5.1.6 0.0053
-
tripolyphosphate G5 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine Escherichia coli
2.5.1.6 0.008
-
tripolyphosphate G8 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine Escherichia coli
2.5.1.6 0.011
-
tripolyphosphate G6 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine Escherichia coli
2.5.1.6 0.014
-
tripolyphosphate RLL mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine Escherichia coli
2.5.1.6 0.015
-
tripolyphosphate G7 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine Escherichia coli
2.5.1.6 0.024
-
tripolyphosphate G5 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine Escherichia coli
2.5.1.6 0.026
-
tripolyphosphate RLL mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine Escherichia coli
2.5.1.6 0.026
-
ATP G6 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.045
-
ATP RLL and G8 mutants, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.069
-
ATP G7 mutants, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.073
-
ATP wild-type S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.087
-
ATP G5 mutant S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.092
-
L-methionine wild-type, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.23
-
L-methionine RLL mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.3
-
L-methionine G6 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.49
-
L-methionine G7 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.62
-
L-methionine G8 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.77
-
L-methionine G5 mutant, S-adenosylmethionine synthesis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Escherichia coli P0A817 XL1-Blue strain
-
2.5.1.6 Escherichia coli XL1-Blue P0A817 XL1-Blue strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 method that includes ammonium sulfate fraction, phenyl-Sepharose HR and hydroxylapetite CHT-1 chromatographies and amminohexyl-Sepharose anion exchange Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
Escherichia coli S-adenosyl-L-methionine + phosphate + diphosphate
-
?
2.5.1.6 ATP + L-methionine + H2O
-
Escherichia coli XL1-Blue S-adenosyl-L-methionine + phosphate + diphosphate
-
?
2.5.1.6 tripolyphosphate + H2O
-
Escherichia coli diphosphate + phosphate
-
?
2.5.1.6 tripolyphosphate + H2O
-
Escherichia coli XL1-Blue diphosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 tetramer gel filtration and native gel electrophoresis Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.6 0.000000217
-
tripolyphosphate G8 mutant, tripolyphosphatase activity Escherichia coli
2.5.1.6 0.000000367
-
S-adenosylmethionine RLL mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.00000045
-
S-adenosylmethionine G6 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.000000617
-
tripolyphosphate G6 mutant, tripolyphosphatase activity Escherichia coli
2.5.1.6 0.000000633
-
tripolyphosphate G5 mutant, tripolyphosphatase activity Escherichia coli
2.5.1.6 0.000000667
-
S-adenosylmethionine G8 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.00000122
-
tripolyphosphate RLL and G7 mutants, tripolyphosphatase activity Escherichia coli
2.5.1.6 0.00000167
-
tripolyphosphate wild-type, tripolyphosphatase activity Escherichia coli
2.5.1.6 0.00000217
-
S-adenosylmethionine G5 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.000012
-
S-adenosylmethionine G7 mutant, S-adenosylmethionine synthesis Escherichia coli
2.5.1.6 0.000417
-
S-adenosylmethionine wild-type, S-adenosylmethionine synthesis Escherichia coli