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Literature summary extracted from

  • Lucker, J.; El Tamer, M.K.; Schwab, W.; Verstappen, F.W.; van der Plas, L.H.; Bouwmeester, H.J.; Verhoeven, H.A.
    Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases (2000), Eur. J. Biochem., 269, 3160-3171.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.20 Mn2+ inhibition above 0.6 mM, activation below Citrus limon

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.20
-
Citrus limon
4.2.3.114 expression in Escherichia coli Citrus limon
4.2.3.120 expression in Escherichia coli Citrus limon

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.20 geranyl diphosphate substrate inhibition above 0.01 mM; substrate inhibition above 0.01 mM Citrus limon
4.2.3.20 K+ maximum inhibition above 100 mM; maximum inhibition above 100 mM Citrus limon
4.2.3.20 Mn2+ above 0.6 mM, activation below; above 0.6 mM, activation below Citrus limon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.20 0.0007
-
geranyl diphosphate
-
Citrus limon
4.2.3.114 0.0027
-
geranyl diphosphate pH 7.0, 30°C Citrus limon
4.2.3.120 0.0031
-
geranyl diphosphate pH 7.0, 30°C Citrus limon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.20 additional information divalent metal ion required, probably Mn2+ Citrus limon
4.2.3.114 Mg2+ or Mn2+, Mn2+ is preferred over Mg2+ Citrus limon
4.2.3.114 Mn2+ or Mg2+, Mn2+ is preferred over Mg2+, optimum concentration 0.6 mM Citrus limon
4.2.3.120 Mg2+ or Mn2+, Mn2+ is preferred over Mg2+ Citrus limon
4.2.3.120 Mn2+ or Mg2+, Mn2+ is preferred over Mg2+, optimum concentration 0.6 mM Citrus limon

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.20 geranyl diphosphate Citrus limon
-
(+)-(R)-limonene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.20 Citrus limon Q8L5K1
-
-
4.2.3.20 Citrus limon Q8L5K3
-
-
4.2.3.114 Citrus limon Q8L5K4
-
-
4.2.3.120 Citrus limon Q8L5K2
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.20 geranyl diphosphate = (R)-limonene + diphosphate requires a divalent metal ion (preferably Mn2+) for catalysis Citrus limon

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.20 fruit peel of young developing fruit Citrus limon
-
4.2.3.114 fruit glants of fruit lavedo and fruit peel Citrus limon
-
4.2.3.120 fruit glants of fruit lavedo and fruit peel Citrus limon
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.20 geranyl diphosphate
-
Citrus limon (+)-(R)-limonene + diphosphate
-
?
4.2.3.20 geranyl diphosphate
-
Citrus limon (+)-(R)-limonene + diphosphate product is exclusively R-(+)-limonene ?
4.2.3.114 geranyl diphosphate
-
Citrus limon gamma-terpinene + diphosphate products are 71.4% gamma-terpinene, with lower amounts of limonene (9.1%), alpha-pinene (5.6%), beta-pinene (4.7%), alpha-terpinolene (3.7%), alpha-thujene (2.5%), alpha-terpinene (1.7%), myrcene (0.9%), sabinene (0.4%) and a trace of p-cymene ?
4.2.3.120 geranyl diphosphate
-
Citrus limon (-)-beta-pinene + diphosphate products are beta-pinene (81.4%, almost exclusively (-)-beta-pinene), sabinene (11%), alpha-pinene (4.1%), limonene (3.5%) and a trace of gamma-terpinene ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.20 7
-
-
Citrus limon