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Literature summary extracted from

  • Wu, T.K.; Busby, R.W.; Houston, T.A.; McIlwaine, D.B.; Egan, L.A.; Townsend, C.A.
    Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis (1995), J. Bacteriol., 177, 3714-3720.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.22
-
 Streptomyces clavuligerus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.22 additional information overexpression as fusion to maltose-binding protein Streptomyces clavuligerus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.22 34000
-
 x * 34000, SDS-PAGE Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.22 guanidinoproclavaminic acid + H2O Streptomyces clavuligerus involved in clavulanic acid biosynthesis proclavaminic acid + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.22 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.22 recombinant enzyme Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.22 deoxyguanidinoproclavaminic acid + H2O
-
 Streptomyces clavuligerus deoxyproclavaminic acid + urea
-
 ?
3.5.3.22 guanidinoproclavaminic acid + H2O best substrate Streptomyces clavuligerus proclavaminic acid + urea
-
 ?
3.5.3.22 guanidinoproclavaminic acid + H2O involved in clavulanic acid biosynthesis Streptomyces clavuligerus proclavaminic acid + urea
-
 ?
3.5.3.22 additional information no substrate inhibition observed, no substrate: D-arginine, L-arginine, N-acetyl-L-arginine Streptomyces clavuligerus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.22 ? x * 34000, SDS-PAGE Streptomyces clavuligerus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.22 37
-
 assay at Streptomyces clavuligerus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.22 9
-
 assay at Streptomyces clavuligerus