EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.78 | HgCl2 | 1 mM, 73% inhibition, addition of 10 mM dithiothreitol restores activity to 100% | Clostridium perfringens | |
3.4.24.78 | additional information | not inhibited by E-64, pepstatin, bestatin, EDTA, antipain, APMSF, leupeptin, aprotinin and SSI | Clostridium perfringens | |
3.4.24.78 | p-chloromercuribenzenesulfonate | 1 mM, 39% inhibition, addition of 10 mM dithiothreitol restores activity to 100% | Clostridium perfringens | |
3.4.24.78 | PMSF | 1 mM, 68% inhibition after 1 h | Clostridium perfringens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.78 | 51442 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
3.4.24.78 | 52000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
3.4.24.78 | 55458 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
3.4.24.78 | 55585 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
3.4.24.78 | 57000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
3.4.24.78 | 60000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.78 | Clostridium perfringens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.78 | Superose 12, hydroxyapatite, DEAE-5PW | Clostridium perfringens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.78 | seed coat | coat of dormant spores | Clostridium perfringens | - |
3.4.24.78 | spore | - |
Clostridium perfringens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.78 | additional information | - |
139363 units, 1 unit is defined as a decrease in the OD600 of 0.1 per min | Clostridium perfringens |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.78 | ? | x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
3.4.24.78 | ? | x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.78 | 4 | - |
72 h, 80% loss of activity in the absence of CHAPS | Clostridium perfringens |
3.4.24.78 | 55 | - |
40 min at pH 7.0, no loss of activity in the presence of 0.1% CHAPS | Clostridium perfringens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.78 | 5.8 | 8.5 | - |
Clostridium perfringens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.78 | 7 | - |
at least 2 month at 4°C in the presence of 0.1% CHAPS, no loss of activity | Clostridium perfringens |