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Literature summary extracted from

  • Raghav, N.; Kamboj, R.C.; Parnami, S.; Singh, H.
    Physico-chemical properties of brain cathepsin H (1995), Indian J. Biochem. Biophys., 32, 279-285.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.16 2-mercaptoethanol activates, synergistic with EDTA Bos taurus
3.4.22.16 dithioerythritol activates, synergistic with EDTA Bos taurus
3.4.22.16 dithiothreitol activates, synergistic with EDTA Bos taurus
3.4.22.16 EDTA activates Bos taurus
3.4.22.16 glutathione activates, synergistic with EDTA Bos taurus
3.4.22.16 thioglycolic acid activates, synergistic with EDTA Bos taurus
3.4.22.16 thiol compounds activate Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.16 2,2'-dipyridyl disulfide 96% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 5,5'-dithiobis(2-nitrobenzoic acid) 95% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 acetyl-(Ala)2-Pro-Ala-CH2Cl 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 acetyl-(Ala)2-Pro-Val-CH2Cl 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 acetyl-(Ala)4-CH2Cl 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 antipain 53% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 bestatin 62% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 decyl bromide 100% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate 90% inhibition at 0.001 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 HgCl2 100% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 iodoacetamide 91% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 iodoacetic acid 100% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 Leu-CH2Cl 92% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 leupeptin 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 MnSO4 64% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 N-ethylmaleimide 98% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 p-Hydroxymercuriphenylsulfonic acid 83% inhibition at 0.011 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 Proteinase inhibitor rat liver Bos taurus
3.4.22.16 puromycin 20% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 thiol-blocking agents
-
 Bos taurus
3.4.22.16 tosyl-Leu-CH2Cl 63% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 tosyl-Phe-CH2Cl 69% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
3.4.22.16 ZnSO4 87% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 0.128
-
 Met-2-naphthylamide
-
 Bos taurus
3.4.22.16 0.167
-
 Leu-2-naphthylamide
-
 Bos taurus
3.4.22.16 0.169
-
 Arg-2-naphthylamide
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.16 25000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.16
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.16 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.16 286
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
 Bos taurus alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
 ir
3.4.22.16 Arg-2-naphthylamide + H2O 97% activity of substrate Leu-2-N-naphthylamine Bos taurus Arg + 2-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Bos taurus Leu + 2-naphthylamine
-
 ir
3.4.22.16 Met-2-naphthylamide + H2O 189% activity of substrate Leu-2-N-naphthylamine Bos taurus Met + 2-naphthylamine
-
 ir

Subunits

EC Number Subunits Comment Organism
3.4.22.16 monomer SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.16 45 50 substrate-dependent Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.16 50
-
 inactivation above Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.16 115
-
 Arg-2-naphthylamide
-
 Bos taurus
3.4.22.16 122
-
 Leu-2-naphthylamide
-
 Bos taurus
3.4.22.16 219
-
 Met-2-naphthylamide
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.16 6.5 7.2 substrate-dependent Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.16 4 7.5
-
 Bos taurus