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Literature summary extracted from
- Purification and characterization of CMP-N-acetylneuraminic acid:lactosylceramide (alpha 2-3) sialyltransferase (GM3-synthase) from rat brain (1993), J. Biol. Chem., 268, 26273-26278.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.3.9 | Triton CF-54 | 0.15%, required for full activity | Rattus norvegicus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.3.9 | Zn2+ | 10 mM, 90% inhibition | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.3.9 | 0.08 | - |
lactosylceramide | - |
Rattus norvegicus | |
| 2.4.3.9 | 0.21 | - |
CMP-N-acetylneuraminate | - |
Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.3.9 | Mn2+ | 10 mM, approx. 2fold activation, required for full activity | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.3.9 | 76000 | - |
x * 76000, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.3.9 | Rattus norvegicus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.3.9 | brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.3.9 | 0.0094 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.3.9 | CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetylgalactosamin-lactosylceramide | 14% of activity with lactosylceramide | Rattus norvegicus | CMP + ? | - |
? | |
| 2.4.3.9 | CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide | i.e. lactosylceramide, high specificity | Rattus norvegicus | CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | i.e. ganglioside GM3 | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.3.9 | ? | x * 76000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.3.9 | 6.5 | - |
- |
Rattus norvegicus |
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