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Literature summary extracted from

  • Wiedemann, M.; Misawa, N.; Sandmann, G.
    Purification and enzymatic characterization of the geranylgeranyl pyrophosphate synthase from Erwinia uredovora after expression in Escherichia coli (1993), Arch. Biochem. Biophys., 306, 152-157.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29 expression in Escherichia coli Pantoea ananatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.009
-
 geranyl diphosphate
-
 Pantoea ananatis
2.5.1.29 0.011
-
 farnesyl diphosphate
-
 Pantoea ananatis
2.5.1.29 0.036
-
 isopentenyl diphosphate
-
 Pantoea ananatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Pantoea ananatis
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Pantoea ananatis
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Pantoea ananatis
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Pantoea ananatis
-
 overexpressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 recombinant enzyme, urea, DEAE-52 Pantoea ananatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Pantoea ananatis geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Pantoea ananatis geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 farnesyl diphosphate + isopentenyl diphosphate
-
 Pantoea ananatis diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Pantoea ananatis geranylgeranyl diphosphate + 2 diphosphate
-
 ?