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Literature summary extracted from

  • Kang, H.; Kang, M.Y.; Han, K.H.
    Identification of natural rubber and characterization of rubber biosynthetic activity in fig tree (2000), Plant Physiol., 123, 1133-1142.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.20 EDTA approx. 3.5fold activation at 20 mM Ficus carica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.20 0.0028
-
farnesyl diphosphate
-
Ficus carica
2.5.1.20 0.228
-
isopentenyl diphosphate
-
Ficus carica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.20 particle-bound
-
Hevea brasiliensis
-
-
2.5.1.20 particle-bound associated with rubber particles Ficus carica
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.20 Mg2+ required for activity Hevea brasiliensis
2.5.1.20 Mg2+ required for activity Ficus carica
2.5.1.20 Mg2+ maximal activity at 1 mM, inhibition above 10 mM Ficus carica
2.5.1.20 Mg2+ maximal activity at 2 mM, inhibition above 10 mM Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.20 Ficus carica
-
-
-
2.5.1.20 Hevea brasiliensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.20 latex
-
Hevea brasiliensis
-
2.5.1.20 latex associated with rubber particles Ficus carica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene
-
Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene
-
Ficus carica diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?
2.5.1.20 isopentenyl diphosphate + farnesyl diphosphate + cis-1,4-polyisoprene an allylic diphosphate e.g. farnesyl diphosphate is required to initiate the biosynthesis of new rubber molecules Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?
2.5.1.20 isopentenyl diphosphate + farnesyl diphosphate + cis-1,4-polyisoprene an allylic diphosphate e.g. farnesyl diphosphate is required to initiate the biosynthesis of new rubber molecules Ficus carica diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?